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PMID: 9861030 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Adenovirus E4orf6 oncoprotein modulates the function of the p53-related protein, p73.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 26 ·1998-12-22 ·Pages 15683-7

Higashino F, Pipas JM, Shenk T

Abstract

Recently, several proteins have been identified that are related in their sequence to the p53 tumor-suppressor protein. One of these proteins, which is termed p73, exhibits sequence homology to the p53 transcriptional activation, DNA binding, and oligomerization domains. The adenovirus E1B 55-kDa protein, the adenovirus E4orf6 protein, and SV40 T antigen each can bind to p53 and inhibit p53 function. Here we demonstrate that the adenovirus E4orf6 protein, but not the E1B 55-kDa protein or T antigen, interacts with p73. The E4orf6 protein inhibits p73-mediated transcriptional activation and cell killing in a manner similar to its effect on p53. Thus, only a subset of viral oncoproteins that antagonize p53 function also interacts with the related p73 protein.

MeSH Terms
Adenoviridae/genetics,physiology Adenovirus E1B Proteins/genetics,metabolism Adenovirus E4 Proteins/genetics,metabolism Animals Cell Line DNA-Binding Proteins/chemistry,genetics,metabolism Genes, Reporter Genes, Tumor Suppressor Humans Luciferases/genetics Mammals Nuclear Proteins/chemistry,genetics,metabolism Recombinant Fusion Proteins/metabolism Transcriptional Activation Transfection Tumor Protein p73 Tumor Suppressor Protein p53/chemistry,metabolism Tumor Suppressor Proteins
Chemicals
Adenovirus E1B Proteins Adenovirus E4 Proteins DNA-Binding Proteins Nuclear Proteins Recombinant Fusion Proteins TP73 protein, human Tumor Protein p73 Tumor Suppressor Protein p53 Tumor Suppressor Proteins Luciferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Higashino F
Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA.
Pipas J M
Shenk T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.26.15683
Published
1998-12-22
Pages
15683-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC28104
Subset
IM
Grants
NCI NIH HHS · P01 CA041086 · United States
NCI NIH HHS · CA41086 · United States
External Links
PubMed source DOI: 10.1073/pnas.95.26.15683 PMC Full Text View journal details
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