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PMID: 9846975 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chemokines IL-8, GROalpha, MCP-1, IP-10, and Mig are sequentially and differentially expressed during phase-specific infiltration of leukocyte subsets in human wound healing.

The American journal of pathology ·Vol. 153 ·No. 6 ·1998-12-00 ·Pages 1849-60

Engelhardt E, Toksoy A, Goebeler M, Debus S, Bröcker EB, Gillitzer R

Abstract

Healing of cutaneous wounds requires a complex integrated network of repair mechanisms, including the action of newly recruited leukocytes. Using a skin repair model in adult humans, we investigated the role chemokines play in sequential infiltration of leukocyte subsets during wound healing. At day 1 after injury, the C-X-C chemokines IL-8 and growth-related oncogene alpha are maximally expressed in the superficial wound bed and are spatially and temporally associated with neutrophil infiltration. IL-8 and growth-related oncogene alpha profiles also correlate with keratinocyte migration and subsequently subside after wound closure at day 4. Macrophage infiltration reaches the highest levels at day 2 and is paralleled by monocyte chemoattractant protein-1 mRNA expression in both the basal layer of the proliferative epidermis at the wound margins and mononuclear cells in the wound area. Other monocyte-attracting chemokines such as monocyte chemoattractant protein-3, macrophage inflammatory protein-1alpha and -1beta, RANTES, and 1309 are undetectable. At day 4, perivascular focal lymphocyte accumulation correlates with strong focal expression of the C-X-C chemokines Mig and IP-10. Our results suggest that a dynamic set of chemokines contributes to the spatially and temporally different infiltration of leukocyte subsets and thus integrates the inflammatory and reparative processes during wound repair.

MeSH Terms
Adult Cell Division Chemokine CCL2/metabolism Chemokine CXCL1 Chemokine CXCL10 Chemokine CXCL9 Chemokines/metabolism Chemokines, CXC/metabolism Chemotactic Factors/metabolism Female Growth Substances/metabolism Humans Immunohistochemistry In Situ Hybridization Intercellular Signaling Peptides and Proteins Interleukin-8/metabolism Keratinocytes/physiology Leukocytes/immunology Male Middle Aged Neovascularization, Physiologic RNA, Messenger/metabolism Time Factors Wound Healing/immunology
Chemicals
CXCL1 protein, human CXCL9 protein, human Chemokine CCL2 Chemokine CXCL1 Chemokine CXCL10 Chemokine CXCL9 Chemokines Chemokines, CXC Chemotactic Factors Growth Substances Intercellular Signaling Peptides and Proteins Interleukin-8 RNA, Messenger
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Engelhardt E
Department of Dermatology, University of Würzburg Medical School, Germany.
Toksoy A
Goebeler M
Debus S
Bröcker E B
Gillitzer R
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/s0002-9440(10)65699-4
Published
1998-12-00
Pages
1849-60
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1866330
Subset
IM
External Links
PubMed source DOI: 10.1016/s0002-9440(10)65699-4 PMC Full Text View journal details
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