Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor.

Biochimica et biophysica acta ·Vol. 1443 ·No. 1-2 ·1998-11-26 ·Pages 245-50

Zhang Y, Spremulli LL

Abstract

The complete sequence of the cDNA for the coding region of human mitochondrial translational release factor 1 has been obtained from human EST clones and 5'RACE. This sequence has been analyzed to provide insights into the relationship between release factors from bacteria and from mammalian mitochondria. The complete cDNA for the human mitochondrial ribosome recycling factor has been assembled using EST clones. This factor has been expressed in Escherichia coli and purified as a His-tagged protein.

MeSH Terms

Amino Acid Sequence DNA, Complementary/genetics Escherichia coli/metabolism Humans Mitochondria/metabolism Mitochondrial Proteins Molecular Sequence Data Peptide Chain Termination, Translational Proteins/chemistry,genetics Ribosomal Proteins Saccharomyces cerevisiae Proteins Sequence Alignment Transcription Factors/chemistry,genetics

Chemicals

DNA, Complementary MTF1 protein, S cerevisiae Mitochondrial Proteins Proteins Ribosomal Proteins Saccharomyces cerevisiae Proteins Transcription Factors ribosome releasing factor

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Zhang Y

Department of Chemistry, Campus Box 3290, University of North Carolina, Chapel Hill, NC 27599-3290, USA.

Spremulli L L

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/s0167-4781(98)00223-1

Published

1998-11-26

Pages

245-50

Language

English

Region

Netherlands

NLM ID

0217513

Subset

Databases

GENBANK

AF072934

External Links

PubMed source DOI: 10.1016/s0167-4781(98)00223-1

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Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor.

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