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PMID: 9826740 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of a murine type II sodium-phosphate cotransporter expressed in mammalian small intestine.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 24 ·1998-11-24 ·Pages 14564-9

Hilfiker H, Hattenhauer O, Traebert M, Forster I, Murer H, Biber J

Abstract

An isoform of the mammalian renal type II Na/Pi-cotransporter is described. Homology of this isoform to described mammalian and nonmammalian type II cotransporters is between 57 and 75%. Based on major diversities at the C terminus, the new isoform is designed as type IIb Na/Pi-cotransporter. Na/Pi-cotransport mediated by the type IIb cotransporter was studied in oocytes of Xenopus laevis. The results indicate that type IIb Na/Pi-cotransport is electrogenic and in contrast to the renal type II isoform of opposite pH dependence. Expression of type IIb mRNA was detected in various tissues, including small intestine. The type IIb protein was detected as a 108-kDa protein by Western blots using isolated small intestinal brush border membranes and by immunohistochemistry was localized at the luminal membrane of mouse enterocytes. Expression of the type IIb protein in the brush borders of enterocytes and transport characteristics suggest that the described type IIb Na/Pi-cotransporter represents a candidate for small intestinal apical Na/Pi-cotransport.

MeSH Terms
Amino Acid Sequence Animals Carrier Proteins/chemistry,genetics,physiology Cattle Female Flounder Genetic Variation Intestinal Mucosa/metabolism Intestine, Small/metabolism Kidney/metabolism Mammals Membrane Potentials/drug effects,physiology Mice Molecular Sequence Data Oocytes/physiology Organ Specificity Phosphates/metabolism,pharmacology Protein Isoforms/chemistry,genetics,physiology RNA, Messenger Sequence Alignment Sequence Homology, Amino Acid Sodium/metabolism,pharmacology Sodium-Phosphate Cotransporter Proteins Sodium-Phosphate Cotransporter Proteins, Type II Sodium-Phosphate Cotransporter Proteins, Type IIb Symporters Transcription, Genetic Xenopus laevis
Chemicals
Carrier Proteins Phosphates Protein Isoforms RNA, Messenger Slc34a2 protein, mouse Sodium-Phosphate Cotransporter Proteins Sodium-Phosphate Cotransporter Proteins, Type II Sodium-Phosphate Cotransporter Proteins, Type IIb Symporters Sodium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hilfiker H
Institut of Physiology, University Zürich, CH-8057 Zurich, Switzerland.
Hattenhauer O
Traebert M
Forster I
Murer H
Biber J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.24.14564
Published
1998-11-24
Pages
14564-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC24413
Subset
IM
Databases
GENBANK
AF081499
External Links
PubMed source DOI: 10.1073/pnas.95.24.14564 PMC Full Text View journal details
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