Home LiteratureArticle Details
PMID: 9811791 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The major resistance gene cluster in lettuce is highly duplicated and spans several megabases.

The Plant cell ·Vol. 10 ·No. 11 ·1998-11-00 ·Pages 1817-32

Meyers BC, Chin DB, Shen KA, Sivaramakrishnan S, Lavelle DO, Zhang Z, Michelmore RW

Abstract

At least 10 Dm genes conferring resistance to the oomycete downy mildew fungus Bremia lactucae map to the major resistance cluster in lettuce. We investigated the structure of this cluster in the lettuce cultivar Diana, which contains Dm3. A deletion breakpoint map of the chromosomal region flanking Dm3 was saturated with a variety of molecular markers. Several of these markers are components of a family of resistance gene candidates (RGC2) that encode a nucleotide binding site and a leucine-rich repeat region. These motifs are characteristic of plant disease resistance genes. Bacterial artificial chromosome clones were identified by using duplicated restriction fragment length polymorphism markers from the region, including the nucleotide binding site-encoding region of RGC2. Twenty-two distinct members of the RGC2 family were characterized from the bacterial artificial chromosomes; at least two additional family members exist. The RGC2 family is highly divergent; the nucleotide identity was as low as 53% between the most distantly related copies. These RGC2 genes span at least 3.5 Mb. Eighteen members were mapped on the deletion breakpoint map. A comparison between the phylogenetic and physical relationships of these sequences demonstrated that closely related copies are physically separated from one another and indicated that complex rearrangements have shaped this region. Analysis of low-copy genomic sequences detected no genes, including RGC2, in the Dm3 region, other than sequences related to retrotransposons and transposable elements. The related but divergent family of RGC2 genes may act as a resource for the generation of new resistance phenotypes through infrequent recombination or unequal crossing over.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Bacterial/genetics Cloning, Molecular DNA Primers/genetics Evolution, Molecular Gene Duplication Gene Rearrangement Genes, Plant Lettuce/genetics,microbiology Molecular Sequence Data Multigene Family Oomycetes/pathogenicity Phylogeny Plant Diseases/genetics,microbiology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length
Chemicals
DNA Primers
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Meyers B C
Department of Vegetable Crops, University of California, Davis, California 95616, USA.
Chin D B
Shen K A
Sivaramakrishnan S
Lavelle D O
Zhang Z
Michelmore R W
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.10.11.1817
Published
1998-11-00
Pages
1817-32
Language
English
Region
England
NLM ID
9208688
PMCID
PMC143960
Subset
IM
Databases
GENBANK
AF072268, AF072271, AF072272, AF072273, AF072274, AF072276, AF072277, AF072278, AF072279, AF072280, AF072281, AF072282, AF072283, AF072284, AF072285, AF072286, AF072287, AF072288, AF113947, AF113948, AF113949, AF113950, AF113953, AF113954, AF113955, AF113956, AF113957
External Links
PubMed source DOI: 10.1105/tpc.10.11.1817 PMC Full Text View journal details
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