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PMID: 9799421 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Perforated patch recording with beta-escin.

Pflugers Archiv : European journal of physiology ·Vol. 436 ·No. 6 ·1998-11-00 ·Pages 1021-3

Fan JS, Palade P

Abstract

Perforated patch recording with nystatin, amphotericin B and gramicidin can be more difficult in the hands of some investigators than others. In addition, it is difficult to introduce low molecular weight substances such as dyes into the cytoplasm in such experiments. We have determined that beta-escin represents a convenient, easy-to-use alternative to less water-soluble ionophores.

MeSH Terms

Amphotericin B Animals Calcium Channels/physiology Electric Conductivity Electric Impedance Escin Fluorescent Dyes Gramicidin Heart/physiology Kinetics Male Nystatin Patch-Clamp Techniques Rats Rats, Sprague-Dawley

Chemicals

Calcium Channels Fluorescent Dyes Nystatin Gramicidin Escin Amphotericin B

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Fan J S

Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas, TX 77555-0641, USA.

Palade P

Article Info

Journal

Pflugers Archiv : European journal of physiology

Abbr.

Pflugers Arch

ISSN

0031-6768

DOI

10.1007/pl00008086

Published

1998-11-00

Pages

1021-3

Language

English

Region

Germany

NLM ID

0154720

Subset

Grants

NIAMS NIH HHS · AR41526 · United States

NIAMS NIH HHS · AR43200 · United States

External Links

PubMed source DOI: 10.1007/pl00008086 View journal details

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Perforated patch recording with beta-escin.

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