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PMID: 9788976 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mast cell activation and migration to lymph nodes during induction of an immune response in mice.

The Journal of clinical investigation ·Vol. 102 ·No. 8 ·1998-10-15 ·Pages 1617-26

Wang HW, Tedla N, Lloyd AR, Wakefield D, McNeil PH

Abstract

The mast cell response in skin and lymph nodes was examined during the sensitization phase of dinitrofluorobenzene (DNFB)-induced contact hypersensitivity in mice. Degranulation of 62% of mast cells in DNFB-exposed skin was evident within 30 min of a dual application of DNFB, reaching a peak of 77% at 24 h, and persisting in 42% after 5 d. Abundant expression of macrophage inflammatory protein (MIP)-1alpha and MIP-1beta mRNAs and proteins was observed in keratinocytes, and mast cell degranulation was significantly inhibited after administration of neutralizing antibodies to MIP-1alpha, but not MIP-1beta. During DNFB sensitization, the mast cell density in the skin decreased by half, concurrent with a fivefold expansion of mast cell numbers in draining lymph nodes. Fluorescent-labeled mast cells injected into the skin appeared in draining lymph nodes after application of DNFB, followed by subsequent migration to the spleen. In lymph nodes, mast cells were an abundant and predominant source of MIP-1beta, neutralization of which partially inhibited T lymphocyte recruitment. These results indicate that mast cells contribute to the induction of this primary immune response by activation at and migration from the site of antigen encounter to draining lymph nodes, wherein they mediate T lymphocyte recruitment by production of MIP-1beta.

MeSH Terms
Animals Cell Degranulation Cell Movement Chemokine CCL3 Chemokine CCL4 Dinitrofluorobenzene/immunology Female Lymph Nodes/immunology Macrophage Inflammatory Proteins/antagonists & inhibitors,biosynthesis Male Mast Cells/immunology Mice Mice, Inbred BALB C Mice, Inbred C3H Skin/immunology Vaccination
Chemicals
Chemokine CCL3 Chemokine CCL4 Macrophage Inflammatory Proteins Dinitrofluorobenzene
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wang H W
Inflammation Research Unit, School of Pathology, University of New South Wales, Sydney, 2052 Australia.
Tedla N
Lloyd A R
Wakefield D
McNeil P H
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI3704
Published
1998-10-15
Pages
1617-26
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC509013
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI3704 PMC Full Text View journal details
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