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PMID: 9774674 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Physical and functional interactions between type I transforming growth factor beta receptors and Balpha, a WD-40 repeat subunit of phosphatase 2A.

Molecular and cellular biology ·Vol. 18 ·No. 11 ·1998-11-00 ·Pages 6595-604

Griswold-Prenner I, Kamibayashi C, Maruoka EM, Mumby MC, Derynck R

Abstract

We have previously shown that a WD-40 repeat protein, TRIP-1, associates with the type II transforming growth factor beta (TGF-beta) receptor. In this report, we show that another WD-40 repeat protein, the Balpha subunit of protein phosphatase 2A, associates with the cytoplasmic domain of type I TGF-beta receptors. This association depends on the kinase activity of the type I receptor, is increased by coexpression of the type II receptor, which is known to phosphorylate and activate the type I receptor, and allows the type I receptor to phosphorylate Balpha. Furthermore, Balpha enhances the growth inhibition activity of TGF-beta in a receptor-dependent manner. Because Balpha has been characterized as a regulator of phosphatase 2A activity, our observations suggest possible functional interactions between the TGF-beta receptor complex and the regulation of protein phosphatase 2A.

MeSH Terms
3T3 Cells Activin Receptors, Type I Animals Cell Division/physiology Cross-Linking Reagents/metabolism Cyclin A/metabolism Gene Expression Regulation, Enzymologic/genetics Genes, Reporter Membrane Proteins/metabolism Mice Phosphoprotein Phosphatases/chemistry Phosphorylation Protein Kinases/metabolism Protein Phosphatase 2 Protein Serine-Threonine Kinases/metabolism Receptor, Transforming Growth Factor-beta Type I Receptor, Transforming Growth Factor-beta Type II Receptors, Transforming Growth Factor beta/metabolism Recombinant Fusion Proteins/metabolism Signal Transduction/physiology
Chemicals
Cross-Linking Reagents Cyclin A Membrane Proteins Receptors, Transforming Growth Factor beta Recombinant Fusion Proteins Protein Kinases Protein Serine-Threonine Kinases Activin Receptors, Type I Receptor, Transforming Growth Factor-beta Type I Receptor, Transforming Growth Factor-beta Type II Phosphoprotein Phosphatases Protein Phosphatase 2
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Griswold-Prenner I
Department of Growth and Development, University of California at San Francisco, San Francisco, California 94143-0640, USA.
Kamibayashi C
Maruoka E M
Mumby M C
Derynck R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.11.6595
Published
1998-11-00
Pages
6595-604
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC109244
Subset
IM
Grants
NCI NIH HHS · CA63101 · United States
NIGMS NIH HHS · GM49505 · United States
External Links
PubMed source DOI: 10.1128/MCB.18.11.6595 PMC Full Text View journal details
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