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PMID: 9774352 Published · ppublish English Journal Article

Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells.

The EMBO journal ·Vol. 17 ·No. 20 ·1998-10-15 ·Pages 6086-95

Salomon S, Puchta H

Abstract

To analyze genomic changes resulting from double-strand break (DSB) repair, transgenic tobacco plants were obtained that carried in their genome a restriction site of the rare cutting endonuclease I-SceI within a negative selectable marker gene. After induction of DSB repair via Agrobacterium-mediated transient expression of I-SceI, plant cells were selected that carried a loss-of-function phenotype of the marker. Surprisingly, in addition to deletions, in a number of cases repair was associated with the insertion of unique and repetitive genomic sequences into the break. Thus, DSB repair offers a mechanism for spreading different kinds of sequences into new chromosomal positions. This may have evolutionary consequences particularly for plants, as genomic alterations occurring in meristem cells can be transferred to the next generation. Moreover, transfer DNA (T-DNA), carrying the open reading frame of I-SceI, was found in several cases to be integrated into the transgenic I-SceI site. This indicates that DSB repair also represents a pathway for the integration of T-DNA into the plant genome.

MeSH Terms
Base Sequence Cytosine Deaminase DNA Damage DNA Repair/genetics,physiology DNA, Bacterial/genetics DNA, Plant/genetics DNA, Single-Stranded/genetics Molecular Sequence Data Mutagenesis, Insertional Nucleoside Deaminases/genetics Plants, Genetically Modified/cytology,genetics Plants, Toxic Rhizobium/genetics Sequence Deletion Tobacco/cytology,genetics
Chemicals
DNA, Bacterial DNA, Plant DNA, Single-Stranded T-DNA Nucleoside Deaminases Cytosine Deaminase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Salomon S
Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany.
Puchta H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.20.6086
Published
1998-10-15
Pages
6086-95
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170935
Subset
IM
Databases
GENBANK
AF061073, AF061074, AF061075, AF061076, AF061077, AF061078
External Links
PubMed source DOI: 10.1093/emboj/17.20.6086 PMC Full Text View journal details
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