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PMID: 9770462 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Efficient recruitment of TFIIB and CBP-RNA polymerase II holoenzyme by an interferon-beta enhanceosome in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 21 ·1998-10-13 ·Pages 12191-6

Kim TK, Kim TH, Maniatis T

Abstract

The transcriptional activity of an in vitro assembled human interferon-beta gene enhanceosome is highly synergistic. This synergy requires five distinct transcriptional activator proteins (ATF2/c-JUN, interferon regulatory factor 1, and p50/p65 of NF-kappaB), the high mobility group protein HMG I(Y), and the correct alignment of protein-binding sites on the face of the DNA double helix. Here, we investigate the mechanisms of enhanceosome-dependent transcriptional synergy during preinitiation complex assembly in vitro. We show that the stereospecific assembly of the enhanceosome is critical for the efficient recruitment of TFIIB into a template-committed TFIID-TFIIA-USA (upstream stimulatory activity complex) and for the subsequent recruitment of the RNA polymerase II holoenzyme complex. In addition, we provide evidence that recruitment of the holoenzyme by the enhanceosome is due, at least in part, to interactions between the enhanceosome and the transcriptional coactivator CREB, cAMP responsive element binding protein (CBP). These studies reveal a unique role of enhanceosomes in the cooperative assembly of the transcription machinery on the human interferon-beta promoter.

MeSH Terms
Cyclic AMP Response Element-Binding Protein/metabolism Enhancer Elements, Genetic HeLa Cells Holoenzymes/metabolism Humans Interferon-beta/genetics Promoter Regions, Genetic RNA Polymerase II/metabolism Transcription Factor TFIIB Transcription Factors/metabolism
Chemicals
Cyclic AMP Response Element-Binding Protein Holoenzymes Transcription Factor TFIIB Transcription Factors Interferon-beta RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim T K
Harvard University, Department of Molecular and Cellular Biology, 7 Divinity Avenue, Cambridge, MA 02138, USA.
Kim T H
Maniatis T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.21.12191
Published
1998-10-13
Pages
12191-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22807
Subset
IM
Grants
NIAID NIH HHS · R01 AI020642 · United States
NIAID NIH HHS · AI20642 · United States
External Links
PubMed source DOI: 10.1073/pnas.95.21.12191 PMC Full Text View journal details
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