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PMID: 9765483 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Epstein-Barr virus small RNA (EBER) genes: differential regulation during lytic viral replication.

Journal of virology ·Vol. 72 ·No. 11 ·1998-11-00 ·Pages 9323-8

Greifenegger N, Jäger M, Kunz-Schughart LA, Wolf H, Schwarzmann F

Abstract

In every latently Epstein-Barr virus-infected cell the viral genes EBER-1 and EBER-2 are transcribed by polymerase III. In lytically infected cells in vivo the EBER genes could not be detected. However, in cell culture downregulation could not be confirmed, and hence the relevance of this shutdown to the replication of the virus was not clear. We assayed the transcriptional activity of the EBER genes by nuclear run-on assays with enriched lytically infected cells and demonstrated that EBER-1 and EBER-2 are differentially downregulated on the transcriptional level during the switch to lytic viral replication. This downregulation was an early event during the lytic replication of the virus.

MeSH Terms
Base Sequence Cell Line DNA Primers/genetics Down-Regulation Gene Expression Regulation, Viral Genes, Viral Herpesvirus 4, Human/genetics,pathogenicity Humans RNA, Viral/genetics Transcription, Genetic Virus Replication/genetics
Chemicals
DNA Primers Epstein-Barr virus encoded RNA 1 Epstein-Barr virus encoded RNA 2 RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Greifenegger N
Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Regensburg, Germany.
Jäger M
Kunz-Schughart L A
Wolf H
Schwarzmann F
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.11.9323-9328.1998
Published
1998-11-00
Pages
9323-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC110355
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.72.11.9323-9328.1998 PMC Full Text View journal details
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