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PMID: 9753324 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A tripartite protein complex with the potential to couple synaptic vesicle exocytosis to cell adhesion in brain.

Cell ·Vol. 94 ·No. 6 ·1998-09-18 ·Pages 773-82

Butz S, Okamoto M, Südhof TC

Abstract

We identify a complex of three proteins in brain that has the potential to couple synaptic vesicle exocytosis to neuronal cell adhesion. The three proteins are: (1) CASK, a protein related to MAGUKs (membrane-associated guanylate kinases); (2) Mint1, a putative vesicular trafficking protein; and (3) Veli1, -2, and -3, vertebrate homologs of C. elegans LIN-7. CASK, Mint1, and Velis form a tight, salt-resistant complex that can be readily isolated. CASK, Mint1, and Velis contain PDZ domains in addition to other modules. However, no PDZ domains are involved in complex formation, leaving them free to recruit cell adhesion molecules, receptors, and channels to the complex. We propose that the tripartite complex acts as a nucleation site for the assembly of proteins involved in synaptic vesicle exocytosis and synaptic junctions.

MeSH Terms

Adaptor Proteins, Signal Transducing Animals Brain Chemistry/physiology Caenorhabditis elegans Caenorhabditis elegans Proteins Calcium-Binding Proteins Calcium-Calmodulin-Dependent Protein Kinases Carrier Proteins/analysis,genetics,metabolism Cell Adhesion/physiology Cell Membrane/chemistry,metabolism Cloning, Molecular Exocytosis/physiology Glycoproteins Guanylate Kinases Helminth Proteins/analysis,genetics,metabolism Humans Membrane Glycoproteins/analysis Membrane Proteins/analysis,genetics,metabolism Mice Molecular Sequence Data Nerve Tissue Proteins/analysis,genetics,metabolism Neurons/chemistry,cytology,physiology Neuropeptides Nucleoside-Phosphate Kinase/analysis,genetics,metabolism Protein Binding/physiology Rats Sequence Homology, Amino Acid Synaptic Vesicles/chemistry,physiology Synaptophysin/analysis Synaptotagmins Tumor Suppressor Proteins Vesicular Transport Proteins

Chemicals

APBA1 protein, human Adaptor Proteins, Signal Transducing Apba1 protein, mouse Apba1 protein, rat Caenorhabditis elegans Proteins Calcium-Binding Proteins Carrier Proteins Glycoproteins Helminth Proteins LIN-7 protein, C elegans LIN7B protein, human Lin7a protein, mouse Lin7b protein, mouse Lin7c protein, mouse Membrane Glycoproteins Membrane Proteins Nerve Tissue Proteins Neuropeptides Synaptophysin Tumor Suppressor Proteins Vesicular Transport Proteins neurexophilin Synaptotagmins neurexin Ibeta CASK kinases Calcium-Calmodulin-Dependent Protein Kinases Nucleoside-Phosphate Kinase DLG2 protein, human Guanylate Kinases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Butz S

Center for Basic Neuroscience, Department of Molecular Genetics, Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, 75235, USA.

Okamoto M

Südhof T C

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/s0092-8674(00)81736-5

Published

1998-09-18

Pages

773-82

Language

English

Region

United States

NLM ID

0413066

Subset

Grants

NIMH NIH HHS · R01-MH52804 · United States

Databases

GENBANK

AF087693, AF087694, AF087695, AF087696, AF087697

External Links

PubMed source DOI: 10.1016/s0092-8674(00)81736-5 View journal details

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A tripartite protein complex with the potential to couple synaptic vesicle exocytosis to cell adhesion in brain.

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