Home LiteratureArticle Details
PMID: 9748443 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of slow growth on metabolism of Escherichia coli, as revealed by global metabolite pool ("metabolome") analysis.

Journal of bacteriology ·Vol. 180 ·No. 19 ·1998-10-00 ·Pages 5109-16

Tweeddale H, Notley-McRobb L, Ferenci T

Abstract

Escherichia coli growing on glucose in minimal medium controls its metabolite pools in response to environmental conditions. The extent of pool changes was followed through two-dimensional thin-layer chromatography of all 14C-glucose labelled compounds extracted from bacteria. The patterns of metabolites and spot intensities detected by phosphorimaging were found to reproducibly differ depending on culture conditions. Clear trends were apparent in the pool sizes of several of the 70 most abundant metabolites extracted from bacteria growing in glucose-limited chemostats at different growth rates. The pools of glutamate, aspartate, trehalose, and adenosine as well as UDP-sugars and putrescine changed markedly. The data on pools observed by two-dimensional thin-layer chromatography were confirmed for amino acids by independent analysis. Other unidentified metabolites also displayed different spot intensities under various conditions, with four trend patterns depending on growth rate. As RpoS controls a number of metabolic genes in response to nutrient limitation, an rpoS mutant was also analyzed for metabolite pools. The mutant had altered metabolite profiles, but only some of the changes at slow growth rates were ascribable to the known control of metabolic genes by RpoS. These results indicate that total metabolite pool ("metabolome") analysis offers a means of revealing novel aspects of cellular metabolism and global regulation.

MeSH Terms
Adenosine/analysis Amino Acids/analysis Bacterial Proteins/genetics,physiology Carbohydrates/analysis Chromatography, Thin Layer/methods Escherichia coli/growth & development,metabolism Glucose/metabolism Glutathione/analysis Mutation Putrescine/analysis Sigma Factor/genetics,physiology
Chemicals
Amino Acids Bacterial Proteins Carbohydrates Sigma Factor sigma factor KatF protein, Bacteria Glutathione Glucose Adenosine Putrescine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tweeddale H
Department of Microbiology, University of Sydney, New South Wales 2006, Australia.
Notley-McRobb L
Ferenci T
References (41)
41 references, click to expand
  1. Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase.
    J Bacteriol. 1975 Jun;122(3):1189-99 PMID: 1097393
  2. [THIN-LAYER CHROMATOGRAPHY OF VARIOUS SUGARS AND SUGAR ALCOHOLS].
    J Chromatogr. 1965 May;18:417-8 PMID: 14318677
  3. The effect of the pH of the medium during growth on the enzymic activities of bacteria (Escherichia coli and Micrococcus lysodeikticus) and the biological significance of the changes produced.
    Biochem J. 1942 Sep;36(7-9):600-18 PMID: 16747564
  4. The role of trehalose in the osmoadaptation of Escherichia coli NCIB 9484: interaction of trehalose, K+ and glutamate during osmoadaptation in continuous culture.
    J Gen Microbiol. 1991 Apr;137(4):745-50 PMID: 1677412
  5. Toward a science of metabolic engineering.
    Science. 1991 Jun 21;252(5013):1668-75 PMID: 2047876
  6. Glutathione biosynthesis in Escherichia coli K 12. Properties of the enzymes and regulation.
    Biochim Biophys Acta. 1975 Jul 14;399(1):1-9 PMID: 238647
  7. Regulation of nitrogen metabolism in Escherichia coli and Klebsiella aerogenes: studies with the continuous-culture technique.
    J Bacteriol. 1975 Aug;123(2):407-18 PMID: 238954
  8. A genetic locus for the GltII-glutamate transport system in Escherichia coli.
    J Gen Microbiol. 1989 Oct;135(10):2767-74 PMID: 2576660
  9. Synthesis and role of glutathione in protection against oxidative stress in yeast.
    Redox Rep. 1996 Aug;2(4):223-9 PMID: 27406271
  10. Polyamines in microorganisms.
    Microbiol Rev. 1985 Mar;49(1):81-99 PMID: 3157043
  11. Levels of glutathione in Escherichia coli.
    Can J Biochem. 1979 Feb;57(2):107-11 PMID: 378330
  12. Dependence of the putrescine content of Escherichia coli on the osmotic strength of the medium.
    J Biol Chem. 1972 Feb 25;247(4):1272-80 PMID: 4551516
  13. Influence of environment on the content and composition of microbial free amino acid pools.
    J Gen Microbiol. 1970 Dec;64(2):171-85 PMID: 4995906
  14. Thin layer chromatographic screening test for amino acid anomalies in urine without desalting using internal standards.
    Clin Chim Acta. 1971 Dec;35(2):445-51 PMID: 5125330
  15. Pool levels of UDP N-acetylglucosamine and UDP N-acetylglucosamine-enolpyruvate in Escherichia coli and correlation with peptidoglycan synthesis.
    J Bacteriol. 1983 Jun;154(3):1284-90 PMID: 6222035
  16. Complete analysis of cellular nucleotides by two-dimensional thin layer chromatography.
    J Biol Chem. 1982 Aug 25;257(16):9759-69 PMID: 6286632
  17. Changes in polyamine levels in various organs of Bombyx mori during its life cycle.
    J Biochem. 1984 Jun;95(6):1803-9 PMID: 6469948
  18. Differential expression of mal genes under cAMP and endogenous inducer control in nutrient-stressed Escherichia coli.
    Mol Microbiol. 1995 Apr;16(1):121-9 PMID: 7651130
  19. Oxygen regulated gene expression in facultatively anaerobic bacteria.
    Antonie Van Leeuwenhoek. 1994;66(1-3):3-22 PMID: 7747938
  20. UDP-glucose is a potential intracellular signal molecule in the control of expression of sigma S and sigma S-dependent genes in Escherichia coli.
    J Bacteriol. 1995 Jan;177(2):413-22 PMID: 7814331
  21. The role of the sigma factor sigma S (KatF) in bacterial global regulation.
    Annu Rev Microbiol. 1994;48:53-80 PMID: 7826018
  22. Between feast and famine: endogenous inducer synthesis in the adaptation of Escherichia coli to growth with limiting carbohydrates.
    J Bacteriol. 1994 Aug;176(16):5101-7 PMID: 8051023
  23. Metabolism and functions of glutathione in micro-organisms.
    Adv Microb Physiol. 1993;34:239-301 PMID: 8095770
  24. The importance of the binding-protein-dependent Mgl system to the transport of glucose in Escherichia coli growing on low sugar concentrations.
    Res Microbiol. 1993 Sep;144(7):529-37 PMID: 8310178
  25. Maltose and maltotriose can be formed endogenously in Escherichia coli from glucose and glucose-1-phosphate independently of enzymes of the maltose system.
    J Bacteriol. 1993 Sep;175(17):5655-65 PMID: 8366051
  26. Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression.
    Mol Microbiol. 1993 Apr;8(2):205-10 PMID: 8391102
  27. Derepression of LamB protein facilitates outer membrane permeation of carbohydrates into Escherichia coli under conditions of nutrient stress.
    J Bacteriol. 1993 Mar;175(5):1475-83 PMID: 8444809
  28. Chromatographic separation of glycated nucleotides.
    J Chromatogr. 1993 Jan 29;612(1):57-61 PMID: 8454703
  29. Entry of Escherichia coli into stationary phase is indicated by endogenous and exogenous accumulation of nucleobases.
    Appl Environ Microbiol. 1995 Dec;61(12):4147-51 PMID: 8534082
  30. How Salmonella survive against the odds.
    Annu Rev Microbiol. 1995;49:145-74 PMID: 8561457
  31. Single-run separation and detection of multiple metabolic intermediates by anion-exchange high-performance liquid chromatography and application to cell pool extracts prepared from Escherichia coli.
    Anal Biochem. 1995 Nov 20;232(1):98-106 PMID: 8600840
  32. Bacterial glutathione: a sacrificial defense against chlorine compounds.
    J Bacteriol. 1996 Apr;178(7):2131-5 PMID: 8606194
  33. Reaction engineering methods to study intracellular metabolite concentrations.
    Adv Biochem Eng Biotechnol. 1996;54:75-108 PMID: 8623615
  34. Induction of RpoS-dependent functions in glucose-limited continuous culture: what level of nutrient limitation induces the stationary phase of Escherichia coli?
    J Bacteriol. 1996 Mar;178(5):1465-8 PMID: 8631726
  35. Adaptation to life at micromolar nutrient levels: the regulation of Escherichia coli glucose transport by endoinduction and cAMP.
    FEMS Microbiol Rev. 1996 Jul;18(4):301-17 PMID: 8703508
  36. EcoCyc: Enyclopedia of Escherichia coli Genes and Metabolism.
    Nucleic Acids Res. 1997 Jan 1;25(1):43-51 PMID: 9016502
  37. Molecular characterization of glucokinase from Escherichia coli K-12.
    J Bacteriol. 1997 Feb;179(4):1298-306 PMID: 9023215
  38. The relationship between external glucose concentration and cAMP levels inside Escherichia coli: implications for models of phosphotransferase-mediated regulation of adenylate cyclase.
    Microbiology. 1997 Jun;143 ( Pt 6):1909-18 PMID: 9202467
  39. Escherichia coli proteome analysis using the gene-protein database.
    Electrophoresis. 1997 Aug;18(8):1243-51 PMID: 9298644
  40. Transcription regulation by initiating NTP concentration: rRNA synthesis in bacteria.
    Science. 1997 Dec 19;278(5346):2092-7 PMID: 9405339
  41. From proteins to proteomes: large scale protein identification by two-dimensional electrophoresis and amino acid analysis.
    Biotechnology (N Y). 1996 Jan;14(1):61-5 PMID: 9636313
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.19.5109-5116.1998
Published
1998-10-00
Pages
5109-16
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107546
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.180.19.5109-5116.1998 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2