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PMID: 9742156 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Basic FGF increases communication between cells of the developing neocortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 18 ·No. 19 ·1998-10-01 ·Pages 7881-90

Nadarajah B, Makarenkova H, Becker DL, Evans WH, Parnavelas JG

Abstract

We have found that basic fibroblast growth factor (bFGF), applied to cortical progenitor cells in vitro, produces an increase in the expression of the gap junction protein connexin (Cx) 43 and in the mRNA encoding Cx 43. This effect was evident in both proliferating and nonproliferating cells. The elevated levels of mRNA suggest that bFGF is likely to exert its effect by upregulating the rate of transcription of the Cx 43 gene. We have further shown that the increase in Cx 43 expression is mediated through the receptor tyrosine kinase pathway and is associated with enhanced intercellular dye-coupling mediated by gap junctions. These results suggest that gap junction channels provide a direct conduit for mitogens released in response to bFGF to effectively regulate proliferation during corticogenesis.

MeSH Terms
Animals Cell Communication/drug effects Cells, Cultured Connexin 43/analysis,genetics Fibroblast Growth Factor 2/pharmacology Fluorescent Dyes Gap Junctions/chemistry Gene Expression/drug effects Isoquinolines Neocortex/cytology Neurons/chemistry,cytology RNA, Messenger/analysis Rats Stem Cells/chemistry,cytology
Chemicals
Connexin 43 Fluorescent Dyes Isoquinolines RNA, Messenger Fibroblast Growth Factor 2 lucifer yellow
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nadarajah B
Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, United Kingdom.
Makarenkova H
Becker D L
Evans W H
Parnavelas J G
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1998-10-01
Pages
7881-90
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6793024
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source PMC Full Text View journal details
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