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PMID: 9740132 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure of 5S rRNA within the Escherichia coli ribosome: iodine-induced cleavage patterns of phosphorothioate derivatives.

RNA (New York, N.Y.) ·Vol. 4 ·No. 9 ·1998-09-00 ·Pages 1154-64

Shpanchenko OV, Dontsova OA, Bogdanov AA, Nierhaus KH

Abstract

The protection patterns of 5S rRNA in solution, within the ribosomal 50S subunit, 70S ribosomes, and functional complexes, were assessed with the phosphorothioate method. About 20% of the analyzed positions (G9-G107) showed strong assembly defects: A phosphorothioate at one of these positions significantly impaired the incorporation of 5S rRNA into 50S particles. The reverse has also been observed: A phosphorothioate is preferred over a phosphate residue in the assembly process at a few positions. The results further demonstrate that 5S rRNA undergoes conformational changes during the assembly in the central protuberance of the 50S subunit and upon association with the small ribosomal subunit forming a 70S ribosome. In striking contrast, when the 70S ribosomes are once formed, the contact pattern of the 5S rRNA is the same in various functional states such as initiation-like complexes and pre- and posttranslocational states.

MeSH Terms
Base Sequence Binding Sites/genetics Escherichia coli/chemistry,genetics,metabolism Iodine Molecular Sequence Data Nucleic Acid Conformation RNA, Bacterial/chemistry,genetics,metabolism RNA, Ribosomal, 5S/chemistry,genetics,metabolism Ribosomes/chemistry,metabolism Thionucleotides/chemistry
Chemicals
RNA, Bacterial RNA, Ribosomal, 5S Thionucleotides Iodine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shpanchenko O V
Department of Chemistry, Moscow State University, Moscow, Russia.
Dontsova O A
Bogdanov A A
Nierhaus K H
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s1355838298980359
Published
1998-09-00
Pages
1154-64
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1369689
Subset
IM
External Links
PubMed source DOI: 10.1017/s1355838298980359 PMC Full Text View journal details
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