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PMID: 9736723 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and characterization of LMO4, an LMO gene with a novel pattern of expression during embryogenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 19 ·1998-09-15 ·Pages 11257-62

Kenny DA, Jurata LW, Saga Y, Gill GN

Abstract

LMO4 is a novel member of the LIM-only (LMO) subfamily of LIM domain-containing transcription factors. LMO1, LMO2, and LMO4 have distinct expression patterns in adult tissue, and we demonstrate that nuclear retention of LMO proteins is enhanced by the nuclear LIM interactor (NLI). In situ hybridization to early mouse embryos of 8-14.5 days revealed a complex pattern of LMO4 expression spatially overlapping with NLI and LHX genes. LMO4 expression in somite is repressed in mice mutant for the segment polarity gene Mesp2 and expanded in Splotch mutants. During jaw and limb outgrowth, LMO4 and LMO2 expression define mesenchyme that is uncommitted to regional fates. Although both LMO2 and LMO4 are activated in thymic blast cells, only LMO4 is expressed in mature T cells. Mesenchymal and thymic blast cell expression patterns of LMO4 and LMO2 are consistent with the suggestion that LMO genes inhibit differentiation.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Cell Differentiation/physiology Cloning, Molecular DNA-Binding Proteins/chemistry,metabolism Embryonic and Fetal Development Gene Expression Regulation, Developmental/genetics Homeodomain Proteins/chemistry Immunohistochemistry In Situ Hybridization LIM Domain Proteins Mice Molecular Sequence Data Nuclear Proteins/chemistry RNA, Messenger/metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid Stem Cells/physiology Transcription Factors/chemistry
Chemicals
Adaptor Proteins, Signal Transducing DNA-Binding Proteins Homeodomain Proteins LDB1 protein, human LIM Domain Proteins Ldb1 protein, mouse Lmo4 protein, mouse Nuclear Proteins RNA, Messenger Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kenny D A
Department of Medicine, University of California at San Diego, La Jolla, CA 92093-0650, USA.
Jurata L W
Saga Y
Gill G N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.19.11257
Published
1998-09-15
Pages
11257-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21629
Subset
IM
Grants
NHLBI NIH HHS · T32 HL007770 · United States
NHLBI NIH HHS · T32HL07770 · United States
NIDDK NIH HHS · R01 DK013149 · United States
NIDDK NIH HHS · DDK13149 · United States
NIDDK NIH HHS · T32 DK007541 · United States
NIDDK NIH HHS · DK07541 · United States
Databases
GENBANK
AF074600
External Links
PubMed source DOI: 10.1073/pnas.95.19.11257 PMC Full Text View journal details
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