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PMID: 9733898 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Optimal induction of hepatitis C virus envelope-specific immunity by bicistronic plasmid DNA inoculation with the granulocyte-macrophage colony-stimulating factor gene.

Journal of virology ·Vol. 72 ·No. 10 ·1998-10-00 ·Pages 8430-6

Lee SW, Cho JH, Sung YC

Abstract

In this study, we have constructed various DNA vaccine vectors that carried hepatitis C virus (HCV) envelope genes without and with the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene in several different ways. In Buffalo rats that received plasmids carrying the HCV envelope genes, which encode envelope proteins E1 and E2, both antibody and lymphoproliferative responses against these proteins were induced. These responses were greatly enhanced by the codelivery of the GM-CSF gene. In particular, inoculation with a bicistronic plasmid that independently expressed the GM-CSF gene and the envelope genes in the same construct generated the highest antibody titers and significantly increased lymphoproliferative responses against these proteins. Moreover, strong antibody responses to homologous and heterologous hypervariable region 1 peptides were elicited in the immunized rats.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Primers Genetic Vectors Granulocyte-Macrophage Colony-Stimulating Factor/genetics Hepacivirus/immunology Hepatitis C Antibodies/biosynthesis Molecular Sequence Data Rats Vaccines, DNA/administration & dosage,genetics Viral Envelope Proteins/immunology
Chemicals
DNA Primers Hepatitis C Antibodies Vaccines, DNA Viral Envelope Proteins Granulocyte-Macrophage Colony-Stimulating Factor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee S W
Department of Life Science, Center for Biofunctional Molecules, School of Environmental Engineering, Pohang University of Science and Technology, Hyoja Dong, Pohang, 790-784 Korea.
Cho J H
Sung Y C
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.10.8430-8436.1998
Published
1998-10-00
Pages
8430-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC110238
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.72.10.8430-8436.1998 PMC Full Text View journal details
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