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PMID: 9712748 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of an acidic-pH-inducible stress protein (hsp70), a putative sulfatide binding adhesin, from Helicobacter pylori.

Infection and immunity ·Vol. 66 ·No. 9 ·1998-09-00 ·Pages 4061-7

Huesca M, Goodwin A, Bhagwansingh A, Hoffman P, Lingwood CA

Abstract

The in vitro glycolipid binding specificity of the gastric pathogen Helicobacter pylori is altered to include sulfated glycolipids (sulfatides) following brief exposure of the organism to acid pH typical of the stomach. This change is prevented by anti-hsp70 antibodies, suggesting that hsp70 may be a stress-induced surface adhesin, mediating sulfatide recognition. To facilitate investigation of the role of hsp70 in attachment, we have cloned and sequenced the H. pylori hsp70 gene (dnaK). The hsp70 gene was identified by probing a cosmid DNA library made from H. pylori 439 with a PCR amplicon generated with oligonucleotides synthesized to highly conserved regions of dnaK. The 1.9-kb H. pylori hsp70 gene encodes a product of 616 amino acids. Primer extension analysis revealed a single transcription start site, while Northern blot analysis established that hsp70 was preferentially induced by low pH rather than by heat shock. The ability of H. pylori to alter its glycolipid binding specificity following exposure to low pH by upregulating hsp70 and by expressing hsp70 on the bacterial surface may provide a survival advantage during periods of high acid stress.

MeSH Terms
Acids Adhesins, Bacterial/genetics,metabolism,physiology Amino Acid Sequence Bacterial Proteins/biosynthesis,genetics,physiology Base Sequence Cloning, Molecular Escherichia coli Proteins Genes, Bacterial HSP70 Heat-Shock Proteins/genetics,metabolism,physiology Hydrogen-Ion Concentration Molecular Sequence Data RNA, Messenger Sequence Homology, Amino Acid Sulfoglycosphingolipids/metabolism
Chemicals
Acids Adhesins, Bacterial Bacterial Proteins Escherichia coli Proteins HSP70 Heat-Shock Proteins RNA, Messenger Sulfoglycosphingolipids adhesin, helicobacter dnaK protein, E coli
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Huesca M
Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.
Goodwin A
Bhagwansingh A
Hoffman P
Lingwood C A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.66.9.4061-4067.1998
Published
1998-09-00
Pages
4061-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC108486
Subset
IM
Databases
GENBANK
AF053125
External Links
PubMed source DOI: 10.1128/IAI.66.9.4061-4067.1998 PMC Full Text View journal details
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