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PMID: 9712747 Published · ppublish English Journal Article

Porphyromonas gingivalis fimbriae use beta2 integrin (CD11/CD18) on mouse peritoneal macrophages as a cellular receptor, and the CD18 beta chain plays a functional role in fimbrial signaling.

Infection and immunity ·Vol. 66 ·No. 9 ·1998-09-00 ·Pages 4056-60

Takeshita A, Murakami Y, Yamashita Y, Ishida M, Fujisawa S, Kitano S, Hanazawa S

Abstract

In this study, we demonstrate that Porphyromonas gingivalis fimbriae use molecules of beta2 integrin (CD11/CD18) on mouse peritoneal macrophages as cellular receptors and also show that the beta chain (CD18) may play a functional role in signalling for the fimbria-induced expression of interleukin-1beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha) genes in the cells. Using a binding assay with 125I-labeled fimbriae, we observed that fimbrial binding to the macrophages was inhibited by treatment with CD11a, CD11b, CD11c, or CD18 antibody but not by that with CD29 antibody. Western blot assays showed that the fimbriae bound to molecules of beta2 integrin (CD11/CD18) on the macrophages. Furthermore, Northern blot analyses showed that the fimbria-induced expression of IL-1beta and TNF-alpha genes in the cells was inhibited strongly by CD18 antibody treatment and slightly by CD11a, CD11b, or CD11c antibody treatment. Interestingly, intracellular adhesion molecule 1 (ICAM-1), a ligand of CD11/CD18, inhibited fimbrial binding to the cells in a dose-dependent manner. In addition, ICAM-1 clearly inhibited the fimbria-induced expression of IL-1beta and TNF-alpha genes in the cells. However, such inhibitory action was not observed with laminin treatment. These results suggest the importance of beta2 integrin (CD11/CD18) as a cellular receptor of P. gingivalis fimbriae in the initiation stage of the pathogenic mechanism of the organism in periodontal disease.

MeSH Terms
Animals Antibodies, Monoclonal/metabolism CD18 Antigens/metabolism,physiology Fimbriae, Bacterial/metabolism Gene Expression Regulation Integrin alphaXbeta2/metabolism Intercellular Adhesion Molecule-1/metabolism Interleukin-1/genetics Ligands Lymphocyte Function-Associated Antigen-1/metabolism Macrophage-1 Antigen/metabolism Macrophages, Peritoneal/metabolism Mice Mice, Inbred BALB C Porphyromonas gingivalis/metabolism Receptors, Cell Surface/metabolism Signal Transduction Tumor Necrosis Factor-alpha/genetics
Chemicals
Antibodies, Monoclonal CD18 Antigens Integrin alphaXbeta2 Interleukin-1 Ligands Lymphocyte Function-Associated Antigen-1 Macrophage-1 Antigen Receptors, Cell Surface Tumor Necrosis Factor-alpha Intercellular Adhesion Molecule-1
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Takeshita A
Departments of Oral Microbiology, Meikai University School of Dentistry, Keyakidai, Sakado City, Saitama 350-0283, Japan.
Murakami Y
Yamashita Y
Ishida M
Fujisawa S
Kitano S
Hanazawa S
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35 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.66.9.4056-4060.1998
Published
1998-09-00
Pages
4056-60
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC108485
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.66.9.4056-4060.1998 PMC Full Text View journal details
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