Comparison of amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism fingerprinting for identification of Acinetobacter genomic species and typing of Acinetobacter baumannii.

Journal of clinical microbiology ·Vol. 36 ·No. 9 ·1998-09-00 ·Pages 2522-9

Koeleman JG, Stoof J, Biesmans DJ, Savelkoul PH, Vandenbroucke-Grauls CM

Abstract

Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting. ARDRA, performed with five different enzymes, showed low discriminatory power for differentiating Acinetobacter at the species and strain level. The standardized commercially available RAPD kit clearly enabled the discrimination of all Acinetobacter genomic species but showed great polymorphism between isolates of Acinetobacter baumannii. AFLP fingerprinting with radioactively as well as fluorescently labelled primers showed high discriminatory power for the identification of 18 Acinetobacter genomic species and typing of 13 clinical Acinetobacter isolates. Compared to radioactive AFLP, fluorescent AFLP was technically fast and simple to perform, and it permitted analysis with an automated DNA sequencer. Fluorescent AFLP seems particularly well suited for studying the epidemiology of nosocomial infections and outbreaks caused by Acinetobacter species.

MeSH Terms

Acinetobacter/classification,genetics,isolation & purification Acinetobacter Infections/diagnosis,microbiology Cross Infection/diagnosis,microbiology DNA Fingerprinting/methods DNA, Bacterial/genetics,isolation & purification Disease Outbreaks Genome, Bacterial Humans Phylogeny Polymerase Chain Reaction/methods Polymorphism, Genetic Random Amplified Polymorphic DNA Technique

Chemicals

DNA, Bacterial

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Koeleman J G

Department of Clinical Microbiology and Infection Control, University Hospital Vrije Universiteit, 1007 MB Amsterdam, The Netherlands.

Stoof J

Biesmans D J

Savelkoul P H

Vandenbroucke-Grauls C M

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/JCM.36.9.2522-2529.1998

Published

1998-09-00

Pages

2522-9

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC105156

Subset

Corrections

CommentIn

PMID: 10515737

External Links

PubMed source DOI: 10.1128/JCM.36.9.2522-2529.1998 PMC Full Text View journal details

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Comparison of amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism fingerprinting for identification of Acinetobacter genomic species and typing of Acinetobacter baumannii.

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