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PMID: 9687513 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

E1A directly binds and regulates the P/CAF acetyltransferase.

The EMBO journal ·Vol. 17 ·No. 15 ·1998-08-03 ·Pages 4469-77

Reid JL, Bannister AJ, Zegerman P, Martínez-Balbás MA, Kouzarides T

Abstract

The P/CAF protein has intrinsic histone acetyltransferase (HAT) activity and is capable of binding the transcriptional co-activator CBP. Here we show that P/CAF can regulate transcription and that this function is independent of its binding to CBP. The HAT domain of P/CAF has transcriptional activation potential in yeast. In mammalian cells P/CAF can stimulate transcription of the RSV promoter, using the activity of its HAT domain. We show that the adenovirus protein E1A targets P/CAF and sequesters its transcriptional activity. Binding of E1A to P/CAF is direct, independent of CBP and requires residues within E1A conserved region 1. We find that the P/CAF binding residues in E1A are within a motif shown to be essential for efficient disruption of myogenesis by E1A. The fact that E1A can directly bind and regulate the activity of P/CAF, independently of its regulation of CBP, highlights an important role for P/CAF in the process of cell differentiation.

MeSH Terms
Acetyltransferases/biosynthesis,metabolism,physiology Adenovirus E1A Proteins/metabolism,physiology CREB-Binding Protein Cell Line Enzyme Activation Enzyme Repression Histone Acetyltransferases Humans Nuclear Proteins/physiology Protein Binding Repressor Proteins/physiology Saccharomyces cerevisiae Proteins Trans-Activators/physiology Transcription, Genetic Tumor Cells, Cultured
Chemicals
Adenovirus E1A Proteins Nuclear Proteins Repressor Proteins Saccharomyces cerevisiae Proteins Trans-Activators Acetyltransferases CREB-Binding Protein CREBBP protein, human Histone Acetyltransferases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reid J L
Wellcome/CRC Institute, Department of Pathology, Cambridge University, UK.
Bannister A J
Zegerman P
Martínez-Balbás M A
Kouzarides T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.15.4469
Published
1998-08-03
Pages
4469-77
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170778
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1093/emboj/17.15.4469 PMC Full Text View journal details
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