Home LiteratureArticle Details
PMID: 9679142 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Translation of the chloroplast psbA mRNA requires the nuclear-encoded poly(A)-binding protein, RB47.

The Journal of cell biology ·Vol. 142 ·No. 2 ·1998-07-27 ·Pages 435-42

Yohn CB, Cohen A, Rosch C, Kuchka MR, Mayfield SP

Abstract

A set of nuclear mutants of C. reinhardtii were identified that specifically lack translation of the chloroplast-encoded psbA mRNA, which encodes the photosystem II reaction center polypeptide D1. Two of these mutants are deficient in the 47-kD member (RB47) of the psbA RNA-binding complex, which has previously been identified both genetically and biochemically as a putative translational activator of the chloroplast psbA mRNA. RB47 is a member of the poly(A)-binding protein family, and binds with high affinity and specificity to the 5' untranslated region of the psbA mRNA. The results presented here confirm RB47's role as a message-specific translational activator in the chloroplast, and bring together genetic and biochemical data to form a cohesive model for light- activated translational regulation in the chloroplast.

MeSH Terms
Animals Cell Nucleus/metabolism Chlamydomonas reinhardtii/genetics,metabolism,radiation effects Chloroplasts/metabolism,radiation effects Gene Expression Light Models, Biological Mutagenesis, Insertional Mutation Photosynthetic Reaction Center Complex Proteins/genetics Photosystem II Protein Complex Poly(A)-Binding Proteins Protein Biosynthesis Protozoan Proteins/genetics,metabolism RNA, Messenger/genetics,metabolism RNA, Protozoan/genetics,metabolism RNA-Binding Proteins/metabolism
Chemicals
Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Poly(A)-Binding Proteins Protozoan Proteins RNA, Messenger RNA, Protozoan RNA-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yohn C B
Department of Cell Biology, and The Skaggs Institute of Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Cohen A
Rosch C
Kuchka M R
Mayfield S P
References (35)
35 references, click to expand
  1. Lack of the D2 protein in a Chlamydomonas reinhardtii psbD mutant affects photosystem II stability and D1 expression.
    EMBO J. 1986 Aug;5(8):1745-54 PMID: 16453694
  2. Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins.
    EMBO J. 1991 Dec;10(13):3993-4001 PMID: 1721866
  3. Proteins encoded by a complex chloroplast transcription unit are each translated from both monocistronic and polycistronic mRNAs.
    EMBO J. 1988 Sep;7(9):2637-44 PMID: 2460341
  4. Low density membranes are associated with RNA-binding proteins and thylakoids in the chloroplast of Chlamydomonas reinhardtii.
    J Cell Biol. 1998 Jan 12;140(1):101-10 PMID: 9425158
  5. A nuclear mutation affects the synthesis of the chloroplast psbA gene production Chlamydomonas reinhardtii.
    Curr Genet. 1992 Jul;22(1):47-52 PMID: 1377098
  6. Protein disulfide isomerase as a regulator of chloroplast translational activation.
    Science. 1997 Dec 12;278(5345):1954-7 PMID: 9395399
  7. Accumulation of D1 polypeptide in tobacco plastids is regulated via the untranslated region of the psbA mRNA.
    EMBO J. 1993 Feb;12(2):601-6 PMID: 8440249
  8. The genetics and cytology of Chlamydomonas.
    Annu Rev Microbiol. 1960;14:197-216 PMID: 13761500
  9. A genetic link between an mRNA-specific translational activator and the translation system in yeast mitochondria.
    Genetics. 1990 Jul;125(3):495-503 PMID: 1696234
  10. A poly(A) binding protein functions in the chloroplast as a message-specific translation factor.
    Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2238-43 PMID: 9482869
  11. Characterization of photosystem II mutants of Chlamydomonas reinhardii lacking the psbA gene.
    Plant Mol Biol. 1986 May;6(3):151-60 PMID: 24307274
  12. ADP-dependent phosphorylation regulates RNA-binding in vitro: implications in light-modulated translation.
    EMBO J. 1994 May 1;13(9):2227-35 PMID: 8187775
  13. Trans-splicing mutants of Chlamydomonas reinhardtii.
    Mol Gen Genet. 1990 Sep;223(3):417-25 PMID: 2270082
  14. Functional interactions among two yeast mitochondrial ribosomal proteins and an mRNA-specific translational activator.
    Genetics. 1991 Feb;127(2):319-26 PMID: 1848523
  15. Nuclear and chloroplast mutations affect the synthesis or stability of the chloroplast psbC gene product in Chlamydomonas reinhardtii.
    EMBO J. 1989 Apr;8(4):1013-21 PMID: 2663467
  16. Interactions among three proteins that specifically activate translation of the mitochondrial COX3 mRNA in Saccharomyces cerevisiae.
    Mol Cell Biol. 1994 Feb;14(2):1045-53 PMID: 8289785
  17. Ribosomal association of poly(A)-binding protein in poly(A)-deficient Saccharomyces cerevisiae.
    J Biol Chem. 1996 May 3;271(18):10859-65 PMID: 8631901
  18. Translation of the psbA mRNA of Chlamydomonas reinhardtii requires a structured RNA element contained within the 5' untranslated region.
    J Cell Biol. 1994 Dec;127(6 Pt 1):1537-45 PMID: 7798310
  19. Translational regulation of gene expression in chloroplasts and mitochondria.
    Annu Rev Genet. 1994;28:71-93 PMID: 7893142
  20. Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G.
    EMBO J. 1996 Dec 16;15(24):7168-77 PMID: 9003792
  21. Translational control of poly(A)-binding protein expression.
    Eur J Biochem. 1996 Apr 1;237(1):143-52 PMID: 8620866
  22. A novel small-subunit ribosomal protein of yeast mitochondria that interacts functionally with an mRNA-specific translational activator.
    Mol Cell Biol. 1990 Sep;10(9):4590-5 PMID: 2167435
  23. The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation.
    Cell. 1989 Sep 8;58(5):857-67 PMID: 2673535
  24. Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis.
    Genetics. 1993 Oct;135(2):375-84 PMID: 8244002
  25. Light-regulated translation of chloroplast messenger RNAs through redox potential.
    Science. 1994 Dec 9;266(5191):1717-9 PMID: 7992056
  26. Comparative analysis of the biogenesis of photosystem II in the wild-type and Y-1 mutant of Chlamydomonas reinhardtii.
    J Cell Biol. 1988 Mar;106(3):609-16 PMID: 3279047
  27. Altered mRNA binding activity and decreased translational initiation in a nuclear mutant lacking translation of the chloroplast psbA mRNA.
    Mol Cell Biol. 1996 Jul;16(7):3560-6 PMID: 8668172
  28. High-frequency nuclear transformation of Chlamydomonas reinhardtii.
    Proc Natl Acad Sci U S A. 1990 Feb;87(3):1228-32 PMID: 2105499
  29. Mutation at the Chlamydomonas nuclear NAC2 locus specifically affects stability of the chloroplast psbD transcript encoding polypeptide D2 of PS II.
    Cell. 1989 Sep 8;58(5):869-76 PMID: 2673536
  30. Post-transcriptional steps in the expression of chloroplast genes.
    Annu Rev Cell Biol. 1992;8:1-28 PMID: 1282351
  31. Analysis of the Saccharomyces cerevisiae mitochondrial COX3 mRNA 5' untranslated leader: translational activation and mRNA processing.
    Mol Cell Biol. 1995 Jun;15(6):3291-300 PMID: 7539105
  32. The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus.
    EMBO J. 1989 Oct;8(10):2803-9 PMID: 2583083
  33. Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photosystem II particles in Chlamydomonas reinhardtii.
    EMBO J. 1987 Feb;6(2):313-8 PMID: 3556163
  34. Autoregulation of poly(A)-binding protein synthesis in vitro.
    Nucleic Acids Res. 1995 Jun 25;23(12):2198-205 PMID: 7610048
  35. Nuclear mutations specifically affect the synthesis and/or degradation of the chloroplast-encoded D2 polypeptide of photosystem II in Chlamydomonas reinhardtii.
    EMBO J. 1988 Feb;7(2):319-24 PMID: 16453828
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.142.2.435
Published
1998-07-27
Pages
435-42
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2133045
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.142.2.435 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2