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PMID: 9671797 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cloning of the Arabidopsis ent-kaurene oxidase gene GA3.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 15 ·1998-07-21 ·Pages 9019-24

Helliwell CA, Sheldon CC, Olive MR, Walker AR, Zeevaart JA, Peacock WJ, Dennis ES

Abstract

The ga3 mutant of Arabidopsis is a gibberellin-responsive dwarf. We present data showing that the ga3-1 mutant is deficient in ent-kaurene oxidase activity, the first cytochrome P450-mediated step in the gibberellin biosynthetic pathway. By using a combination of conventional map-based cloning and random sequencing we identified a putative cytochrome P450 gene mapping to the same location as GA3. Relative to the progenitor line, two ga3 mutant alleles contained single base changes generating in-frame stop codons in the predicted amino acid sequence of the P450. A genomic clone spanning the P450 locus complemented the ga3-2 mutant. The deduced GA3 protein defines an additional class of cytochrome P450 enzymes. The GA3 gene was expressed in all tissues examined, RNA abundance being highest in inflorescence tissue.

MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics Base Sequence Cloning, Molecular Cytochrome P-450 Enzyme System/genetics DNA, Recombinant Gene Expression Regulation Genetic Complementation Test Molecular Sequence Data Mutation Oxygenases/genetics
Chemicals
DNA, Recombinant Cytochrome P-450 Enzyme System Oxygenases ent-kaurene oxidase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Helliwell C A
Commonwealth Scientific and Industrial Research Organization, Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia.
Sheldon C C
Olive M R
Walker A R
Zeevaart J A
Peacock W J
Dennis E S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.15.9019
Published
1998-07-21
Pages
9019-24
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21195
Subset
IM
Databases
GENBANK
AF047719, AF047720, AF047721
External Links
PubMed source DOI: 10.1073/pnas.95.15.9019 PMC Full Text View journal details
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