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PMID: 965488 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Urate crystal-induced chemotactic factor: isolation and partial characterization.

The Journal of clinical investigation ·Vol. 58 ·No. 4 ·1976-10-00 ·Pages 815-9

Spilbert I, Gallacher A, Mehta JM, Mandell B

Abstract

A factor with chemotactic properties for neutrophils and mononuclear cells was extracted from the lysosomal fraction of both human and rabbit neutrophils that had been allowed to phagocytose monosodium urate crystals. The chemotactic factor was found to be a glycoprotein with a mol wt of 8,400 daltons. The factor is heat labile and has chemotactic activity for human as well as rabbit cells. Preincubation of the cells with the urate induced chemotactic factor or with complement activated plasma prevents the cell from migrating chemotactically when challenged with either factor in the chemotactic chamber. The chemotactic factor induces release of lysosomal enzymes for cytochalasin B treated human neutrophils.

MeSH Terms
Glucuronidase Glycoproteins/isolation & purification,pharmacology L-Lactate Dehydrogenase Molecular Weight Neutrophils/enzymology Phagocytosis Uric Acid
Chemicals
Glycoproteins Uric Acid L-Lactate Dehydrogenase Glucuronidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Spilbert I
Gallacher A
Mehta J M
Mandell B
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22 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI108533
Published
1976-10-00
Pages
815-9
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC333243
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI108533 PMC Full Text View journal details
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