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PMID: 965475 Published · ppublish English Comparative Study Journal Article

Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.

Journal of clinical microbiology ·Vol. 4 ·No. 2 ·1976-08-00 ·Pages 117-23

Kalimo KO, Meurman OH, Halonen PE, Ziola BR, Viljanen MK, Granfors K, Toivanen P

Abstract

A solid-phase radioimmunoassay method has been developed for the detection of rubella virus-specific immunoglobulin G (IgG) and IgM antibodies in human serum specimens. Purified rubella virus was adsorbed onto polystyrene balls, and antibodies that attached to the virus-treated balls were detected by subsequent binding of 125I-labeled anti-human gamma or anti-human mu immunoglobulins. A total of 77 serum specimens were tested. Binding ratios between positive and negative sera were as high as 22 in the IgG assay but rarely exceeded 3 in the IgM assay. The sensitivity of the IgG assay was found to be 16 to 256 times higher than that of the rubella virus hemagglutination inhibition test. The IgG radioimmunoassay can be readily adopted for routine diagnostic use. The IgM radioimmunoassay, however, due to its lower sensitivity, must be modified before being routinely applied.

MeSH Terms
Antibodies, Viral/analysis Complement Fixation Tests Diagnosis, Differential Evaluation Studies as Topic Hemagglutination Inhibition Tests Humans Immunoglobulin G/analysis Immunoglobulin M/analysis Iodine Radioisotopes Polystyrenes Radioimmunoassay/methods Rubella/diagnosis Rubella virus/immunology
Chemicals
Antibodies, Viral Immunoglobulin G Immunoglobulin M Iodine Radioisotopes Polystyrenes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kalimo K O
Meurman O H
Halonen P E
Ziola B R
Viljanen M K
Granfors K
Toivanen P
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.4.2.117-123.1976
Published
1976-08-00
Pages
117-23
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC274410
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.4.2.117-123.1976 PMC Full Text View journal details
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