Design and Application of Two Rapid Screening Techniques for Isolation of Mn(IV) Reduction-Deficient Mutants of Shewanella putrefaciens.

Applied and environmental microbiology ·Vol. 64 ·No. 7 ·1998-07-01 ·Pages 2716-20

Burnes BS, Mulberry MJ, DiChristina TJ

Abstract

Chemical mutagenesis procedures and two newly developed rapid plate assays were used to identify two Mn(IV) reduction-deficient (Mnr) mutants of Shewanella putrefaciens. All eleven members of a set of previously isolated Fe(III) reduction-deficient (Fer) mutants displayed Mnr-positive phenotypes on the plate assays and were also capable of anaerobic growth on Mn(IV) as the sole terminal electron acceptor.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Burnes

School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332.

Mulberry

DiChristina

References (15)

15 references, click to expand

Cloning and sequence of cymA, a gene encoding a tetraheme cytochrome c required for reduction of iron(III), fumarate, and nitrate by Shewanella putrefaciens MR-1.
J Bacteriol. 1997 Feb;179(4):1143-52 PMID: 9023196
Design and application of rRNA-targeted oligonucleotide probes for the dissimilatory iron- and manganese-reducing bacterium Shewanella putrefaciens.
Appl Environ Microbiol. 1993 Dec;59(12):4152-60 PMID: 7506899
Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese.
Appl Environ Microbiol. 1988 Jun;54(6):1472-80 PMID: 16347658
Respiration-linked proton translocation coupled to anaerobic reduction of manganese(IV) and iron(III) in Shewanella putrefaciens MR-1.
J Bacteriol. 1990 Nov;172(11):6232-8 PMID: 2172208
Hydrogen and Formate Oxidation Coupled to Dissimilatory Reduction of Iron or Manganese by Alteromonas putrefaciens.
Appl Environ Microbiol. 1989 Mar;55(3):700-6 PMID: 16347876
Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor.
Science. 1988 Jun 3;240(4857):1319-21 PMID: 17815852
Effect of nitrate on reduction of ferric iron by a bacterium isolated from crude oil.
Can J Microbiol. 1981 Jul;27(7):692-7 PMID: 7197577
Sulfate reduction and oxic respiration in marine sediments: implications for organic carbon preservation in euxinic environments.
Deep Sea Res A. 1989;36(1):121-38 PMID: 11542177
Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals.
Arch Microbiol. 1993;159(4):336-44 PMID: 8387263
Isolation of Geobacter species from diverse sedimentary environments.
Appl Environ Microbiol. 1996 May;62(5):1531-6 PMID: 8633852
Ferric iron reduction-linked growth yields of Shewanella putrefaciens MR-1.
J Appl Bacteriol. 1994 Mar;76(3):253-8 PMID: 8157545
Dissimilatory Fe(III) and Mn(IV) reduction.
Microbiol Rev. 1991 Jun;55(2):259-87 PMID: 1886521
Isolation of anaerobic respiratory mutants of Shewannella putrefaciens and genetic analysis of mutants deficient in anaerobic growth on Fe3+.
J Bacteriol. 1994 Mar;176(5):1468-74 PMID: 8113188
Iron and manganese in anaerobic respiration: environmental significance, physiology, and regulation.
Annu Rev Microbiol. 1994;48:311-43 PMID: 7826009
Microbial reduction of manganese and iron: new approaches to carbon cycling.
Appl Environ Microbiol. 1992 Feb;58(2):439-43 PMID: 1610166

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

1098-5336

DOI

10.1128/AEM.64.7.2716-2720.1998

Published

1998-07-01

Pages

2716-20

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC106451

External Links

PubMed source DOI: 10.1128/AEM.64.7.2716-2720.1998 PMC Full Text View journal details

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Design and Application of Two Rapid Screening Techniques for Isolation of Mn(IV) Reduction-Deficient Mutants of Shewanella putrefaciens.

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