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PMID: 9635740 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Spontaneous-curvature theory of clathrin-coated membranes.

Biophysical journal ·Vol. 74 ·No. 6 ·1998-06-00 ·Pages 2862-75

Mashl RJ, Bruinsma RF

Abstract

Clathrin-coated membranes are precursors to coated vesicles in the receptor-mediated endocytic pathway. In this paper we present a physical model for the first steps of the transformation of a clathrin-coated membrane into a coated vesicle. The theory is based on in vitro cytoplasmic acidification experiments of Heuser (J. Cell Biol. 108:401-411) that suggest the transformation proceeds by changes in the chemical environment of the clathrin lattice, wherein the chemical environment determines the amount of intrinsic, or spontaneous, curvature of the network. We show that a necessary step of the transformation, formation of free pentagons in the clathrin network, can proceed via dislocation unbinding, driven by changes in the spontaneous curvature. Dislocation unbinding is shown to favor formation of coated vesicles that are quite small compared to those predicted by the current continuum theories, which do not include the topology of the clathrin lattice.

MeSH Terms
Animals Clathrin/physiology,ultrastructure Coated Pits, Cell-Membrane/physiology,ultrastructure Cytoplasm/physiology Elasticity Endocytosis Kinetics Lipid Bilayers Membranes, Artificial Microscopy, Electron Models, Biological Models, Theoretical Thermodynamics
Chemicals
Clathrin Lipid Bilayers Membranes, Artificial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mashl R J
Department of Chemistry and Biochemistry, University of California at Los Angeles, 90095, USA. mashl@lanl.gov
Bruinsma R F
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(98)77993-7
Email
mashl@lanl.gov
Published
1998-06-00
Pages
2862-75
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1299627
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(98)77993-7 PMC Full Text View journal details
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