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PMID: 9632758 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction of TATA-binding protein with upstream activation factor is required for activated transcription of ribosomal DNA by RNA polymerase I in Saccharomyces cerevisiae in vivo.

Molecular and cellular biology ·Vol. 18 ·No. 7 ·1998-07-00 ·Pages 3752-61

Steffan JS, Keys DA, Vu L, Nomura M

Abstract

Previous in vitro studies have shown that initiation of transcription of ribosomal DNA (rDNA) in the yeast Saccharomyces cerevisiae involves an interaction of upstream activation factor (UAF) with the upstream element of the promoter, forming a stable UAF-template complex; together with TATA-binding protein (TBP), UAF then recruits an essential factor, core factor (CF), to the promoter, forming a stable preinitiation complex. TBP interacts with both UAF and CF in vitro. In addition, a subunit of UAF, Rrn9p, interacts with TBP in vitro and in the two-hybrid system, suggesting the possible importance of this interaction for UAF function. Using the yeast two-hybrid system, we have identified three mutations in RRN9 that abolish the interaction of Rrn9p with TBP without affecting its interaction with Rrn10p, another subunit of UAF. Yeast cells containing any one of these individual mutations, L110S, L269P, or L274Q, did not show any growth defects. However, cells containing a combination of L110S with one of the other two mutations showed a temperature-sensitive phenotype, and this phenotype was suppressed by fusing the mutant genes to SPT15, which encodes TBP. In addition, another mutation (F186S), which disrupts both Rrn9p-TBP and Rrn9p-Rrn10p interactions in the two-hybrid system, abolished UAF function in vivo, and this mutational defect was suppressed by fusion of the mutant gene to SPT15 combined with overexpression of Rrn10p. These experiments demonstrate that the interaction of UAF with TBP, which is presumably achieved by the interaction of Rrn9p with TBP, is indeed important for high-level transcription of rDNA by RNA polymerase I in vivo.

MeSH Terms
Artificial Gene Fusion Binding Sites DNA, Fungal/genetics DNA, Ribosomal/genetics DNA-Binding Proteins/genetics,metabolism Genes, Fungal Mutagenesis Nucleic Acid Hybridization RNA Polymerase I/metabolism RNA Polymerase II/metabolism Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/enzymology,genetics,metabolism Saccharomyces cerevisiae Proteins TATA-Box Binding Protein Trans-Activators/metabolism Transcription Factors/genetics,metabolism Transcriptional Activation
Chemicals
DNA, Fungal DNA, Ribosomal DNA-Binding Proteins RRN10 protein, S cerevisiae RRN9 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins TATA-Box Binding Protein Trans-Activators Transcription Factors RNA Polymerase II RNA Polymerase I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Steffan J S
Department of Biological Chemistry, University of California-Irvine, Irvine, California 92697-1700, USA.
Keys D A
Vu L
Nomura M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.7.3752
Published
1998-07-00
Pages
3752-61
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC108958
Subset
IM
Grants
NIGMS NIH HHS · R37GM35949 · United States
External Links
PubMed source DOI: 10.1128/MCB.18.7.3752 PMC Full Text View journal details
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