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PMID: 9618496 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The gastrulation defective gene of Drosophila melanogaster is a member of the serine protease superfamily.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 12 ·1998-06-09 ·Pages 6819-24

Konrad KD, Goralski TJ, Mahowald AP, Marsh JL

Abstract

The establishment of dorsal-ventral polarity in the oocyte involves two sets of genes. One set belongs to the gurken-torpedo signaling pathway and affects the development of the egg chorion as well as the polarity of the embryo. The second set of genes affects only the dorsal-ventral polarity of the embryo but not the eggshell. gastrulation defective is one of the earliest acting of this second set of maternally required genes. We have cloned and characterized the gastrulation defective gene and determined that it encodes a protein structurally related to the serine protease superfamily, which also includes the Snake, Easter, and Nudel proteins. These data provide additional support for the involvement of a protease cascade in generating an asymmetric signal (i.e., asymmetric Spätzle activity) during establishment of dorsal-ventral polarity in the Drosophila embryo.

MeSH Terms
Amino Acid Sequence Animals Cloning, Molecular Drosophila melanogaster/embryology,genetics Gene Expression Regulation, Developmental Genes, Insect Insect Proteins/genetics Molecular Sequence Data Sequence Alignment Serine Endopeptidases/genetics
Chemicals
Insect Proteins Serine Endopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Konrad K D
Developmental Biology Center and the Department of Developmental and Cell Biology, University of California Irvine, Irvine, CA 92697-2300, USA.
Goralski T J
Mahowald A P
Marsh J L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.12.6819
Published
1998-06-09
Pages
6819-24
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22648
Subset
IM
Grants
NIGMS NIH HHS · GM28972 · United States
NICHD NIH HHS · HD17607 · United States
NICHD NIH HHS · P01 HD27173 · United States
Databases
GENBANK
U09808
External Links
PubMed source DOI: 10.1073/pnas.95.12.6819 PMC Full Text View journal details
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