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PMID: 9600981 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of the phenylacetic acid catabolic pathway in Pseudomonas putida U: the phenylacetyl-CoA catabolon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 11 ·1998-05-26 ·Pages 6419-24

Olivera ER, Miñambres B, García B, Muñiz C, Moreno MA, Ferrández A, Díaz E, García JL, Luengo JM

Abstract

Fourteen different genes included in a DNA fragment of 18 kb are involved in the aerobic degradation of phenylacetic acid by Pseudomonas putida U. This catabolic pathway appears to be organized in three contiguous operons that contain the following functional units: (i) a transport system, (ii) a phenylacetic acid activating enzyme, (iii) a ring-hydroxylation complex, (iv) a ring-opening protein, (v) a beta-oxidation-like system, and (vi) two regulatory genes. This pathway constitutes the common part (core) of a complex functional unit (catabolon) integrated by several routes that catalyze the transformation of structurally related molecules into a common intermediate (phenylacetyl-CoA).

MeSH Terms
Acetyl Coenzyme A/genetics,metabolism Amino Acid Sequence Biodegradation, Environmental Molecular Sequence Data Mutation Phenylacetates/metabolism Pseudomonas putida/metabolism Sequence Alignment
Chemicals
Phenylacetates Acetyl Coenzyme A phenylacetyl-coenzyme A phenylacetic acid
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Olivera E R
Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, 24007 León, Spain.
Miñambres B
García B
Muñiz C
Moreno M A
Ferrández A
Díaz E
García J L
Luengo J M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.11.6419
Published
1998-05-26
Pages
6419-24
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC27761
Subset
IM
Databases
GENBANK
AF029714
External Links
PubMed source DOI: 10.1073/pnas.95.11.6419 PMC Full Text View journal details
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