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PMID: 9592138 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RNA heptamers that direct RNA cleavage by mammalian tRNA 3' processing endoribonuclease.

Nucleic acids research ·Vol. 26 ·No. 11 ·1998-06-01 ·Pages 2565-72

Nashimoto M, Geary S, Tamura M, Kaspar R

Abstract

Mammalian tRNA 3' processing endoribonuclease (3' tRNase) can recognize and cleave any target RNA that forms a precursor tRNA-like complex with another RNA. Various sets of RNA molecules were tested to identify the smallest RNA that can direct target RNA cleavage by 3' tRNase. A 3' half tRNAArgwas cleaved efficiently by 3' tRNase in the presence of small 5' half tRNAArgvariants, the D stem-loop region of which was partially deleted. Remarkably, 3' tRNase also cleaved the 3' half tRNAArgin the presence of a 7 nt 5' tRNAArg composed only of the acceptor stem region with a catalytic efficiency comparable with that of cleavage directed by an intact 5' half tRNAArg. The catalytic efficiency of cleavage directed by the heptamer decreased as the stability of the T stem-loop structures of 3' half tRNAArg variants decreased. No heptamer-directed cleavage of a 3' half tRNAArg without T stem base pairs was detected. A heptamer also directed cleavage of an HIV-1 RNA containing a stable hairpin structure. These findings suggest that in the presence of an RNA heptamer, 3' tRNase can discriminate and eliminate target RNAs that possess a stable hairpin adjacent to the heptamer binding sequence from a large complex RNA pool.

MeSH Terms

Animals Base Sequence Cell Line Endoribonucleases/metabolism,physiology Genes, env Genes, gag HIV-1/genetics Mice Molecular Sequence Data Nucleic Acid Conformation RNA, Transfer, Arg/metabolism RNA, Viral/metabolism Substrate Specificity

Chemicals

RNA, Transfer, Arg RNA, Viral Endoribonucleases tRNA 3'-processing endonuclease

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Nashimoto M

Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA. mnashimoto@chemgate.byu.edu

Geary S

Tamura M

Kaspar R

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/26.11.2565

mnashimoto@chemgate.byu.edu

Published

1998-06-01

Pages

2565-72

Language

English

Region

England

NLM ID

0411011

PMCID

PMC147582

Subset

External Links

PubMed source DOI: 10.1093/nar/26.11.2565 PMC Full Text View journal details

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RNA heptamers that direct RNA cleavage by mammalian tRNA 3' processing endoribonuclease.

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