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PMID: 9584149 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

H2-M3 restricted presentation of a Listeria-derived leader peptide.

The Journal of experimental medicine ·Vol. 187 ·No. 10 ·1998-05-18 ·Pages 1711-9

Princiotta MF, Lenz LL, Bevan MJ, Staerz UD

Abstract

Protective immunity to infection by many intracellular pathogens requires recognition by cytotoxic T lymphocytes (CTLs) of antigens presented on major histocompatibility complex (MHC) class I molecules. To be presented for recognition by pathogen-specific CTLs, these antigens must gain access to the host cell class I processing pathway. In the case of intracellular bacterial pathogens, the majority of bacterial proteins are retained within the bacterial membrane and therefore remain inaccessible to the host cell for antigen processing. We have isolated a CTL clone from a C57BL/6 mouse infected with the intracellular gram-positive bacterium Listeria monocytogenes (LM) and have identified the source of the antigen. Using a genomic expression library, we determined that the clone recognizes an antigenic N-formyl peptide presented by the nonpolymorphic murine MHC class Ib molecule, H2-M3. Several lengths of this peptide were able to sensitize cells for lysis by this CTL clone. The source of this antigenic peptide is a 23-amino acid polypeptide encoded at the start of a polycistronic region. Analysis of mRNA secondary structure of this region suggests that this polypeptide may be a leader peptide encoded by a transcriptional attenuator.

MeSH Terms
Amino Acid Sequence Animals Antigen Presentation Antigens, Bacterial/immunology Base Sequence Histocompatibility Antigens Class I/immunology Listeria monocytogenes/immunology Listeriosis/immunology Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred DBA Molecular Sequence Data Peptides/immunology T-Lymphocytes, Cytotoxic/immunology,microbiology
Chemicals
Antigens, Bacterial Histocompatibility Antigens Class I Peptides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Princiotta M F
National Jewish Medical and Research Center, University of Colorado Health Sciences Center, Denver, Colorado 80206, USA.
Lenz L L
Bevan M J
Staerz U D
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.187.10.1711
Published
1998-05-18
Pages
1711-9
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2212287
Subset
IM
Grants
NIAID NIH HHS · AI-35194 · United States
NIAID NIH HHS · AI-22295 · United States
NICHD NIH HHS · HD-26841 · United States
Databases
GENBANK
AF039207
External Links
PubMed source DOI: 10.1084/jem.187.10.1711 PMC Full Text View journal details
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