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PMID: 9580703 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

DNA condensation for gene therapy as monitored by atomic force microscopy.

Nucleic acids research ·Vol. 26 ·No. 10 ·1998-05-15 ·Pages 2481-7

Hansma HG, Golan R, Hsieh W, Lollo CP, Mullen-Ley P, Kwoh D

Abstract

The atomic force microscope (AFM) was used to assay the extent of DNA condensation in approximately 100 different complexes of DNA with polylysine (PL) or PL covalently attached to the glycoproteins asialoorosomucoid (AsOR) or orosomucoid (OR). The best condensation of DNA was obtained with 10 kDa PL covalently attached to AsOR, at a lysine:nucleotide (Lys:nt) ratio of 5:1 or higher. These conditions produce large numbers of toroids and short rods with contour lengths of 300-400 nm. Some DNA condensation into shortened thickened structures was seen with 10 kDa PL attached to AsOR at Lys:nt ratios of 1.6:1 and 3:1. Some DNA condensation was also seen with 4 kDa PL at Lys:nt ratios of 3:1 and higher. Little DNA condensation was seen with PL alone or with PL convalently attached to OR at Lys:nt ratios up to 6:1. AsOR-PL enhanced gene expression in the mouse liver approximately 10- to 50-fold as compared with PL alone.

MeSH Terms
Animals Asialoglycoproteins DNA/genetics,ultrastructure Gene Transfer Techniques Liver/metabolism Luciferases/genetics Mice Mice, Inbred BALB C Microscopy, Atomic Force/methods Orosomucoid/analogs & derivatives Plasmids Polylysine
Chemicals
Asialoglycoproteins Orosomucoid asialoorosomucoid Polylysine DNA Luciferases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hansma H G
Department of Physics, University of California, Santa Barbara, CA 93106, USA. hhansma@physics.ucsb.edu
Golan R
Hsieh W
Lollo C P
Mullen-Ley P
Kwoh D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/26.10.2481
Email
hhansma@physics.ucsb.edu
Published
1998-05-15
Pages
2481-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147577
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/26.10.2481 PMC Full Text View journal details
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