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PMID: 9573181 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The 102-kilobase unstable region of Yersinia pestis comprises a high-pathogenicity island linked to a pigmentation segment which undergoes internal rearrangement.

Journal of bacteriology ·Vol. 180 ·No. 9 ·1998-05-00 ·Pages 2321-9

Buchrieser C, Prentice M, Carniel E

Abstract

Several pathogenicity islands have recently been identified in different bacterial species, including a high-pathogenicity island (HPI) in Yersinia enterocolitica 1B. In Y. pestis, a 102-kb chromosomal fragment (pgm locus) that carries genes involved in iron acquisition and colony pigmentation can be deleted en bloc. In this study, characterization and mapping of the 102-kb region of Y. pestis 6/69 were performed to determine if this unstable region is a pathogenicity island. We found that the 102-kb region of Y. pestis is composed of two clearly distinct regions: an approximately 35-kb iron acquisition segment, which is an HPI per se, linked to an approximately 68-kb pigmentation segment. This linkage was preserved in all of the Y. pestis strains studied. However, several nonpigmented Y. pestis strains harboring an irp2 gene have been previously identified, suggesting that the pigmentation segment is independently mobile. Comparison of the physical map of the 102-kb region of these strains with that of strain 6/69 and complementation experiments were carried out to determine the genetic basis of this phenomenon. We demonstrate that several different mechanisms involving mutations and various-size deletions are responsible for the nonpigmented phenotype in the nine strains studied. However, no deletion corresponded exactly to the pigmentation segment. The 102-kb region of Y. pestis is an evolutionarily stable linkage of an HPI with a pigmentation segment in a region of the chromosome prone to rearrangement in vitro.

MeSH Terms
Bacterial Outer Membrane Proteins Bacterial Proteins Chromosome Walking Chromosomes, Bacterial Cosmids Democratic Republic of the Congo Genetic Linkage Germany Humans Iron/metabolism Iron-Binding Proteins Kenya Madagascar Molecular Epidemiology Periplasmic Binding Proteins Pigments, Biological Plague/epidemiology Polymerase Chain Reaction Recombination, Genetic Repetitive Sequences, Nucleic Acid Restriction Mapping Vietnam Yersinia pestis/genetics,pathogenicity
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Iron-Binding Proteins Periplasmic Binding Proteins Pigments, Biological Iron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Buchrieser C
Unité de Bactériologie Moléculaire et Médicale, Laboratoire des Yersinia, Institut Pasteur, Paris, France.
Prentice M
Carniel E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.9.2321-2329.1998
Published
1998-05-00
Pages
2321-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107171
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.180.9.2321-2329.1998 PMC Full Text View journal details
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