Home LiteratureArticle Details
PMID: 9566907 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Ash1, a daughter cell-specific protein, is required for pseudohyphal growth of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 18 ·No. 5 ·1998-05-00 ·Pages 2884-91

Chandarlapaty S, Errede B

Abstract

Ash1 (for asymmetric synthesis of HO) was first uncovered in genetic screens that revealed its role in mating-type switching. Ash1 prevents HO expression in daughter cells. Because Ash1 has a zinc finger-like domain related to that of the GATA family of transcription factors, it presumably acts by repressing HO transcription. Nonswitching diploid cells also express Ash1, suggesting it could have functions in addition to regulation of HO expression. We show here that Ash1 has an essential function for pseudohyphal growth. Our epistasis analyses are consistent with the deduction that Ash1 acts separately from the mitogen-activated protein kinase cascade and Ste12. Similarly to the case in yeast form cells, Ash1 is asymmetrically localized to the nuclei of daughter cells during pseudohyphal growth. This asymmetric localization reveals that there is a previously unsuspected daughter cell-specific function necessary for pseudohyphal growth.

MeSH Terms
Cell Compartmentation Cell Differentiation DNA-Binding Proteins Epistasis, Genetic Fungal Proteins/metabolism Models, Biological Morphogenesis/genetics Phenotype Repressor Proteins Saccharomyces cerevisiae/cytology,growth & development Saccharomyces cerevisiae Proteins Signal Transduction Transcription Factors/genetics,isolation & purification,metabolism Zinc Fingers
Chemicals
ASH1 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins Repressor Proteins STE12 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chandarlapaty S
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-7260, USA.
Errede B
References (35)
35 references, click to expand
  1. Regulating the HO endonuclease in yeast.
    Curr Opin Genet Dev. 1993 Apr;3(2):286-94 PMID: 8504254
  2. GATA-binding transcription factors in hematopoietic cells.
    Blood. 1992 Aug 1;80(3):575-81 PMID: 1638017
  3. Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development.
    Mol Cell Biol. 1994 Mar;14(3):2100-12 PMID: 8114741
  4. Involvement of SRE element of Ty1 transposon in TEC1-dependent transcriptional activation.
    Nucleic Acids Res. 1994 Mar 25;22(6):999-1005 PMID: 8152932
  5. MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1994 May;14(5):3139-49 PMID: 8164669
  6. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth.
    Genes Dev. 1994 Dec 15;8(24):2974-85 PMID: 8001818
  7. Symmetric cell division in pseudohyphae of the yeast Saccharomyces cerevisiae.
    Mol Biol Cell. 1994 Sep;5(9):1003-22 PMID: 7841518
  8. Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae.
    J Cell Biol. 1995 Aug;130(3):687-700 PMID: 7622568
  9. SOK2 may regulate cyclic AMP-dependent protein kinase-stimulated growth and pseudohyphal development by repressing transcription.
    Mol Cell Biol. 1995 Dec;15(12):6854-63 PMID: 8524252
  10. Budding yeast morphogenesis: signalling, cytoskeleton and cell cycle.
    Curr Opin Cell Biol. 1995 Dec;7(6):845-55 PMID: 8608015
  11. Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins.
    Cell. 1996 Mar 8;84(5):687-97 PMID: 8625407
  12. Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells.
    Cell. 1996 Mar 8;84(5):699-709 PMID: 8625408
  13. Identification of asymmetrically localized determinant, Ash1p, required for lineage-specific transcription of the yeast HO gene.
    Cell. 1996 Mar 8;84(5):711-22 PMID: 8625409
  14. Ras2 signals via the Cdc42/Ste20/mitogen-activated protein kinase module to induce filamentous growth in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1996 May 28;93(11):5352-6 PMID: 8643578
  15. SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells.
    Microbiology. 1996 Oct;142 ( Pt 10):2897-905 PMID: 8885406
  16. Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae.
    Genes Dev. 1996 Nov 15;10(22):2831-48 PMID: 8918885
  17. Functional analysis of the interaction between the small GTP binding protein Cdc42 and the Ste20 protein kinase in yeast.
    EMBO J. 1996 Dec 16;15(24):7046-59 PMID: 9003780
  18. Identification of a mid-anaphase checkpoint in budding yeast.
    J Cell Biol. 1997 Jan 27;136(2):345-54 PMID: 9015305
  19. Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae.
    Genetics. 1997 Mar;145(3):671-84 PMID: 9055077
  20. Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates.
    Curr Biol. 1997 Apr 1;7(4):228-38 PMID: 9094309
  21. 14-3-3 proteins are essential for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae.
    Cell. 1997 Jun 27;89(7):1055-65 PMID: 9215628
  22. Cooperative binding interactions required for function of the Ty1 sterile responsive element.
    Mol Cell Biol. 1997 Aug;17(8):4330-7 PMID: 9234690
  23. Gpa2p, a G-protein alpha-subunit, regulates growth and pseudohyphal development in Saccharomyces cerevisiae via a cAMP-dependent mechanism.
    J Biol Chem. 1997 Aug 15;272(33):20321-3 PMID: 9252333
  24. Nonfilamentous C. albicans mutants are avirulent.
    Cell. 1997 Sep 5;90(5):939-49 PMID: 9298905
  25. Yeast pseudohyphal growth is regulated by GPA2, a G protein alpha homolog.
    EMBO J. 1997 Dec 1;16(23):7008-18 PMID: 9384580
  26. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains.
    Genetics. 1987 Aug;116(4):541-5 PMID: 3305158
  27. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  28. The yeast STE12 protein binds to the DNA sequence mediating pheromone induction.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5703-7 PMID: 2668945
  29. STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast.
    Genes Dev. 1990 Nov;4(11):1862-74 PMID: 2276621
  30. Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.
    Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1731-5 PMID: 1848010
  31. High-efficiency transformation of yeast by electroporation.
    Methods Enzymol. 1991;194:182-7 PMID: 2005786
  32. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
    Methods Enzymol. 1991;194:281-301 PMID: 2005793
  33. Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS.
    Cell. 1992 Mar 20;68(6):1077-90 PMID: 1547504
  34. Improved method for high efficiency transformation of intact yeast cells.
    Nucleic Acids Res. 1992 Mar 25;20(6):1425 PMID: 1561104
  35. Elements of the yeast pheromone response pathway required for filamentous growth of diploids.
    Science. 1993 Dec 10;262(5140):1741-4 PMID: 8259520
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.5.2884
Published
1998-05-00
Pages
2884-91
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC110667
Subset
IM
Grants
NIGMS NIH HHS · GM-39852 · United States
External Links
PubMed source DOI: 10.1128/MCB.18.5.2884 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2