Home LiteratureArticle Details

PMID: 9557754 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Processing of the Borna disease virus glycoprotein gp94 by the subtilisin-like endoprotease furin.

Journal of virology ·Vol. 72 ·No. 5 ·1998-05-00 ·Pages 4528-33

Richt JA, Fürbringer T, Koch A, Pfeuffer I, Herden C, Bause-Niedrig I, Garten W

Abstract

Open reading frame IV (ORF-IV) of Borna disease virus (BDV) encodes a protein with a calculated molecular mass of ca. 57 kDa (p57), which increases after N glycosylation to 94 kDa (gp94). The unglycosylated and glycosylated proteins are proteolytically cleaved by the subtilisin-like protease furin. Furin most likely recognizes one of three potential cleavage sites, namely, an arginine at position 249 of the ORF-IV gene product. The furin inhibitor decRVKRcmk decreases the production of infectious BDV significantly, indicating that proteolytic cleavage of the gp94 precursor molecule is necessary for the full biological activity of the BDV glycoprotein.

MeSH Terms

Amino Acid Chloromethyl Ketones/pharmacology Amino Acid Sequence Animals Borna disease virus/metabolism,physiology Brain/metabolism Cell Line Chlorocebus aethiops Furin Glycoproteins/chemistry,metabolism Glycosylation Protein Processing, Post-Translational Rats Serine Proteinase Inhibitors/pharmacology Subtilisins/metabolism Viral Proteins/chemistry,metabolism

Chemicals

Amino Acid Chloromethyl Ketones Glycoproteins Serine Proteinase Inhibitors Viral Proteins Subtilisins Furin

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Richt J A

Institut für Virologie, Giessen, Germany. juergen.a.richt@vetmed.uni-giessen.de

Fürbringer T

Koch A

Pfeuffer I

Herden C

Bause-Niedrig I

Garten W

References (23)

23 references, click to expand

Influenza virus hemagglutinin with multibasic cleavage site is activated by furin, a subtilisin-like endoprotease.
EMBO J. 1992 Jul;11(7):2407-14 PMID: 1628614
Detection of a novel Borna disease virus-encoded 10 kDa protein in infected cells and tissues.
J Gen Virol. 1997 Oct;78 ( Pt 10):2459-66 PMID: 9349465
Carbohydrate masking of an antigenic epitope of influenza virus haemagglutinin independent of oligosaccharide size.
Glycobiology. 1992 Jun;2(3):233-40 PMID: 1379858
Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gp160.
Nature. 1992 Nov 26;360(6402):358-61 PMID: 1360148
Biology and neurobiology of Borna disease viruses (BDV), defined by antibodies, neutralizability and their pathogenic potential.
Arch Virol Suppl. 1993;7:111-33 PMID: 8219797
Sequence and genome organization of Borna disease virus.
J Virol. 1994 Mar;68(3):1382-96 PMID: 7906311
Molecular biology of borna disease virus: prototype of a new group of animal viruses.
J Virol. 1994 Dec;68(12):7669-75 PMID: 7966555
Maturation of the trans-Golgi network protease furin: compartmentalization of propeptide removal, substrate cleavage, and COOH-terminal truncation.
J Cell Biol. 1994 Dec;127(6 Pt 2):1829-42 PMID: 7806563
Immunopathogenesis of Borna disease.
Curr Top Microbiol Immunol. 1995;190:75-92 PMID: 7789151
The remarkable coding strategy of borna disease virus: a new member of the nonsegmented negative strand RNA viruses.
Virology. 1995 Jun 20;210(1):1-8 PMID: 7793061
Similar structural models of the transmembrane proteins of Ebola and avian sarcoma viruses.
Cell. 1996 May 17;85(4):477-8 PMID: 8653783
Biochemical and functional analysis of the Borna disease virus G protein.
J Virol. 1997 Jan;71(1):331-6 PMID: 8985354
The role of eukaryotic subtilisin-like endoproteases for the activation of human immunodeficiency virus glycoproteins in natural host cells.
J Virol. 1997 Feb;71(2):1036-45 PMID: 8995623
Characterization of Borna disease virus p56 protein, a surface glycoprotein involved in virus entry.
J Virol. 1997 Apr;71(4):3208-18 PMID: 9060684
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Replication of Borna disease virus in cell cultures.
Med Microbiol Immunol. 1980;168(3):153-8 PMID: 6772932
General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes.
J Virol. 1984 Mar;49(3):857-64 PMID: 6321770
Purification and properties of an intranuclear virus-specific antigen from tissue infected with Borna disease virus.
J Gen Virol. 1986 Feb;67 ( Pt 2):235-41 PMID: 3080548
The structure of serotype H10 hemagglutinin of influenza A virus: comparison of an apathogenic avian and a mammalian strain pathogenic for mink.
Virology. 1988 Aug;165(2):428-37 PMID: 3407149
The molecular biology of influenza virus pathogenicity.
Adv Virus Res. 1988;34:247-81 PMID: 3046255
Inhibition of proteolytic activation of influenza virus hemagglutinin by specific peptidyl chloroalkyl ketones.
Virology. 1989 Sep;172(1):25-31 PMID: 2773317
Infection with Borna disease virus: molecular and immunobiological characterization of the agent.
Clin Infect Dis. 1992 Jun;14(6):1240-50 PMID: 1623080
Consensus sequence for precursor processing at mono-arginyl sites. Evidence for the involvement of a Kex2-like endoprotease in precursor cleavages at both dibasic and mono-arginyl sites.
J Biol Chem. 1992 Aug 15;267(23):16335-40 PMID: 1644818

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.72.5.4528-4533.1998

juergen.a.richt@vetmed.uni-giessen.de

Published

1998-05-00

Pages

4528-33

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC109700

Subset

External Links

PubMed source DOI: 10.1128/JVI.72.5.4528-4533.1998 PMC Full Text View journal details

Analysis Services

Processing of the Borna disease virus glycoprotein gp94 by the subtilisin-like endoprotease furin.

Contact

WeChat Official Account