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PMID: 9557746 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Patterns of CCR5, CXCR4, and CCR3 usage by envelope glycoproteins from human immunodeficiency virus type 1 primary isolates.

Journal of virology ·Vol. 72 ·No. 5 ·1998-05-00 ·Pages 4485-91

Bazan HA, Alkhatib G, Broder CC, Berger EA

Abstract

Coreceptor usage by Envs from diverse primary human immunodeficiency virus type 1 isolates was analyzed by a vaccinia virus-based expression and assay system. Usage of recombinant CCR5 and CXCR4 correlated closely with fusogenicity toward macrophages and T-cell lines expressing endogenous coreceptors. Surprisingly, recombinant CCR3 was utilized by most primary and T-cell-line-adapted Envs. Endogenous CXCR4 in macrophages was functional as a coreceptor.

MeSH Terms
HIV-1/genetics,metabolism Humans Macrophages/metabolism Membrane Fusion Receptors, CCR3 Receptors, CCR5/metabolism Receptors, CXCR4/metabolism Receptors, Chemokine/metabolism Viral Envelope Proteins/genetics,metabolism
Chemicals
CCR3 protein, human Receptors, CCR3 Receptors, CCR5 Receptors, CXCR4 Receptors, Chemokine Viral Envelope Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bazan H A
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0445, USA.
Alkhatib G
Broder C C
Berger E A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.5.4485-4491.1998
Published
1998-05-00
Pages
4485-91
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC109686
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.72.5.4485-4491.1998 PMC Full Text View journal details
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