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PMID: 9555909 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Oxygen regulation of the ccoN gene encoding a component of the cbb3 oxidase in Rhodobacter sphaeroides 2.4.1T: involvement of the FnrL protein.

Journal of bacteriology ·Vol. 180 ·No. 8 ·1998-04-00 ·Pages 2228-31

Mouncey NJ, Kaplan S

Abstract

The ccoNOQP gene cluster of Rhodobacter sphaeroides 2.4.1T encodes a cbb3 cytochrome oxidase which is utilized in oxygen-limited conditions for aerobic respiration. The beta-galactosidase activity of a ccoN::lacZ transcriptional fusion was low under high (30%)-oxygen and anaerobic growth conditions. Maximal ccoN::lacZ expression was observed when the oxygen concentration was lowered to 2%. In an FnrL mutant, ccoN::lacZ expression was significantly lower than in the wild-type strain, suggesting that FnrL is a positive regulator of genes encoding the cbb3 oxidase.

MeSH Terms
Aerobiosis Anaerobiosis Bacterial Proteins/metabolism Base Sequence Electron Transport Complex IV/biosynthesis,genetics Escherichia coli/genetics,growth & development Gene Expression Regulation, Bacterial/drug effects,physiology Genes, Bacterial Multigene Family Oxygen/pharmacology Oxygen Consumption Recombinant Fusion Proteins/biosynthesis Rhodobacter sphaeroides/enzymology,genetics Trans-Activators Transcription, Genetic beta-Galactosidase/biosynthesis
Chemicals
Bacterial Proteins FnrL protein, Rhodobacter sphaeroides Recombinant Fusion Proteins Trans-Activators Electron Transport Complex IV beta-Galactosidase Oxygen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mouncey N J
Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center Medical School, Houston 77030, USA.
Kaplan S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.8.2228-2231.1998
Published
1998-04-00
Pages
2228-31
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107153
Subset
IM
Grants
NIGMS NIH HHS · R01 GM015590 · United States
NIGMS NIH HHS · GM15590 · United States
External Links
PubMed source DOI: 10.1128/JB.180.8.2228-2231.1998 PMC Full Text View journal details
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