A novel subtype of G-protein-coupled receptor kinase, GRK7, in teleost cone photoreceptors.

Two kinds of retinal cDNA fragments (OIGRK-R and -C) encoding the putative G-protein-coupled receptor kinases (GRKs) were isolated from medaka, Oryzias latipes. OIGRK-R appears to be closely related to the rhodopsin kinase (RK) found in the outer segments of mammalian photoreceptors, but the deduced amino acid sequence of OIGRK-C shows less than 50% identity to those of GRKs known to date, suggesting that OIGRK-C is a novel GRK subtype (GRK7). The mRNA of OIGRK-R is detectable in rods, and that of OIGRK-C is found in all four types of cone photoreceptor. The C-terminal of OIGRK-R has a consensus sequence for farnesylation, whereas, surprisingly, OIGRK-C has a consensus sequence for geranylgeranylation. Our result are consistent with the concept that lower vertebrates have rod- and cone-specific opsin kinases.