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PMID: 9536051 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cloning and characterization of AtRGP1. A reversibly autoglycosylated arabidopsis protein implicated in cell wall biosynthesis.

Plant physiology ·Vol. 116 ·No. 4 ·1998-04-00 ·Pages 1339-50

Delgado IJ, Wang Z, de Rocher A, Keegstra K, Raikhel NV

Abstract

A reversibly glycosylated polypeptide from pea (Pisum sativum) is thought to have a role in the biosynthesis of hemicellulosic polysaccharides. We have investigated this hypothesis by isolating a cDNA clone encoding a homolog of Arabidopsis thaliana, Reversibly Glycosylated Polypeptide-1 (AtRGP1), and preparing antibodies against the protein encoded by this gene. Polyclonal antibodies detect homologs in both dicot and monocot species. The patterns of expression and intracellular localization of the protein were examined. AtRGP1 protein and RNA concentration are highest in roots and suspension-cultured cells. Localization of the protein shows it to be mostly soluble but also peripherally associated with membranes. We confirmed that AtRGP1 produced in Escherichia coli could be reversibly glycosylated using UDP-glucose and UDP-galactose as substrates. Possible sites for UDP-sugar binding and glycosylation are discussed. Our results are consistent with a role for this reversibly glycosylated polypeptide in cell wall biosynthesis, although its precise role is still unknown.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,metabolism Arabidopsis Proteins Cell Wall/metabolism Cloning, Molecular DNA, Complementary Escherichia coli/genetics Glycoproteins/genetics,metabolism Glycosylation Membrane Proteins/genetics,metabolism Molecular Sequence Data Plant Proteins/genetics,metabolism Polysaccharides/biosynthesis RNA, Messenger/genetics,metabolism Sequence Homology, Amino Acid Solubility
Chemicals
Arabidopsis Proteins DNA, Complementary Glycoproteins Membrane Proteins Plant Proteins Polysaccharides RGP1 protein, Arabidopsis RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Delgado I J
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing 48824-1312, USA.
Wang Z
de Rocher A
Keegstra K
Raikhel N V
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.116.4.1339
Published
1998-04-00
Pages
1339-50
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC35041
Subset
IM
Databases
GENBANK
AF013627, AF013628, AF034255
External Links
PubMed source DOI: 10.1104/pp.116.4.1339 PMC Full Text View journal details
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