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PMID: 9525652 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vivo replication capacity rather than in vitro macrophage tropism predicts efficiency of vaginal transmission of simian immunodeficiency virus or simian/human immunodeficiency virus in rhesus macaques.

Journal of virology ·Vol. 72 ·No. 4 ·1998-04-00 ·Pages 3248-58

Miller CJ, Marthas M, Greenier J, Lu D, Dailey PJ, Lu Y

Abstract

We used the rhesus macaque model of heterosexual human immunodeficiency virus (HIV) transmission to test the hypothesis that in vitro measures of macrophage tropism predict the ability of a primate lentivirus to initiate a systemic infection after intravaginal inoculation. A single atraumatic intravaginal inoculation with a T-cell-tropic molecular clone of simian immunodeficiency virus (SIV), SIVmac239, or a dualtropic recombinant molecular clone of SIV, SIVmac239/1A11/239, or uncloned dualtropic SIVmac251 or uncloned dualtropic simian/human immunodeficiency virus (SHIV) 89.6-PD produced systemic infection in all rhesus macaques tested. However, vaginal inoculation with a dualtropic molecular clone of SIV, SIVmac1A11, resulted in transient viremia in one of two rhesus macaques. It has previously been shown that 12 intravaginal inoculations with SIVmac1A11 resulted in infection of one of five rhesus macaques (M. L. Marthas, C. J. Miller, S. Sutjipto, J. Higgins, J. Torten, B. L. Lohman, R. E. Unger, H. Kiyono, J. R. McGhee, P. A. Marx, and N. C. Pedersen, J. Med. Primatol. 21:99-107, 1992). In addition, SHIV HXBc2, which replicates in monkey macrophages, does not infect rhesus macaques following multiple vaginal inoculations, while T-cell-tropic SHIV 89.6 does (Y. Lu, P. B. Brosio, M. Lafaile, J. Li, R. G. Collman, J. Sodroski, and C. J. Miller, J. Virol. 70:3045-3050, 1996). These results demonstrate that in vitro measures of macrophage tropism do not predict if a SIV or SHIV will produce systemic infection after intravaginal inoculation of rhesus macaques. However, we did find that the level to which these viruses replicate in vivo after intravenous inoculation predicts the outcome of intravaginal inoculation with each virus.

MeSH Terms
Animals Antigens, Viral/blood Cells, Cultured Cloning, Molecular Female HIV-1/physiology Humans Injections, Intravenous Leukocytes, Mononuclear/virology Macaca mulatta Macrophages/virology Simian Immunodeficiency Virus/genetics,growth & development,physiology Vagina/virology Viral Load Virus Replication
Chemicals
Antigens, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Miller C J
California Regional Primate Research Center, and Department of Veterinary Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis 95616, USA. cjmiller@ucdavis.edu
Marthas M
Greenier J
Lu D
Dailey P J
Lu Y
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.4.3248-3258.1998
Email
cjmiller@ucdavis.edu
Published
1998-04-00
Pages
3248-58
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC109796
Subset
IM
Grants
NCRR NIH HHS · P51 RR000169 · United States
NIAID NIH HHS · AI35545 · United States
NIAID NIH HHS · AI39109 · United States
NCRR NIH HHS · RR00169 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1128/JVI.72.4.3248-3258.1998 PMC Full Text View journal details
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