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PMID: 95177 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Centrifugal elutriation (counterstreaming centrifugation) of cells.

Cell biophysics ·Vol. 1 ·No. 2 ·1979-06-00 ·Pages 195-210

Pretlow TG, Pretlow TP

Abstract

Lindahl first described the separation of cells by velocity sedimentation utilizing a special technique (counterstreaming centrifugation) that was later modified slightly and renamed centrifugal elutriation. Centrifugal elutriation has been applied, with variable degrees of success, to the separation of hemopoietic cells, mouse tumor cells, testicular cells, and a variety of other specialized cells as well as cells in particular phases of the cell cycle. The capacity of the elutriator to separate large numbers of cells is its chief advantage. The purities of the separated cells have not been compared with the purities of cells separated by other methods in most cases; such comparisons would permit more sophisticated comparison of elutriation with other techniques for velocity sedimentation.

MeSH Terms
Animals Brain/cytology Cell Cycle Cell Line Cell Separation/methods Cell Transformation, Neoplastic Centrifugation/methods Erythrocytes/cytology Eukaryota/cytology Humans Kupffer Cells/cytology Leukocytes/cytology Male Mast Cells/cytology Neoplasms/pathology Testis/cytology
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pretlow T G
Pretlow T P
References (48)
48 references, click to expand
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Article Info
Journal
Cell biophysics
Abbr.
Cell Biophys
ISSN
0163-4992
DOI
10.1007/BF02781351
Published
1979-06-00
Pages
195-210
Language
English
Region
United States
NLM ID
8002185
Subset
IM
Grants
NCI NIH HHS · CA-13148 · United States
NCI NIH HHS · CA-23922 · United States
External Links
PubMed source DOI: 10.1007/BF02781351
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