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PMID: 9499799 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evolution of human immunodeficiency virus type 1 envelope sequences in infected individuals with differing disease progression profiles.

Virology ·Vol. 241 ·No. 2 ·1998-02-15 ·Pages 251-9

Shankarappa R, Gupta P, Learn GH, Rodrigo AG, Rinaldo CR, Gorry MC, Mullins JI, Nara PL, Ehrlich GD

Abstract

Sequence variation displayed by the human immunodeficiency virus type 1 (HIV-1) has been proposed to be linked to the pathogenesis of acquired immunodeficiency syndrome (AIDS). To assess viral evolution during the course of infection, we evaluated sequence variability in the env variable domains in four HIV-1-infected individuals exhibiting differing profiles of CD4+ T cell decline when followed from seroconversion until the development of AIDS or loss of followup. Proviral sequences encoding the V3-V5 region of gp 120 were obtained following PCR amplification of peripheral blood mononuclear cell DNA and cloning. Virus in each patient was relatively homogeneous early in infection and then diverged with time, more consistently at its nonsynonymous sites. Just prior to or coincident with a rapid decline in CD4+ T cell numbers, sequences were found with basic amino acid substitutions clustered within and downstream of the gp 120 V3 domain. Within the constraints of the current data set, we conclude that the virus appears to continually accumulate changes in its amino acid sequences well into the time of marked CD4+ T cell decline.

MeSH Terms
Amino Acid Sequence Base Sequence CD4 Lymphocyte Count Cohort Studies DNA, Viral Disease Progression Follow-Up Studies Genetic Variation HIV Envelope Protein gp120/classification,genetics HIV Infections/immunology,physiopathology,virology HIV-1/classification,genetics Humans Male Molecular Sequence Data Mutagenesis Peptide Fragments/classification,genetics Phylogeny Sequence Homology, Amino Acid Time Factors
Chemicals
DNA, Viral HIV Envelope Protein gp120 HIV envelope protein gp120 (305-321) Peptide Fragments
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Shankarappa R
Department of Pathology, University of Pittsburgh, Pennsylvania 15261, USA.
Gupta P
Learn G H
Rodrigo A G
Rinaldo C R
Gorry M C
Mullins J I
Nara P L
Ehrlich G D
Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1006/viro.1997.8996
Published
1998-02-15
Pages
251-9
Language
English
Region
United States
NLM ID
0110674
Subset
IM
Grants
NIAID NIH HHS · AI34783 · United States
NIAID NIH HHS · N01-AI-72632 · United States
NIAID NIH HHS · U0-1-AI-35041 · United States
Databases
GENBANK
U15804, U15805, U15806, U15807, U15808, U15809, U15810, U15811, U15812, U15813, U15814, U15815, U15816, U15817, U15818, U15819, U15820, U15821, U15822, U15823, U15824, U15825, U15826, U15827, U15828, U15829, U15830, U15831, U15832, U15833
External Links
PubMed source DOI: 10.1006/viro.1997.8996 View journal details
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