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PMID: 9490789 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

NF-kappaB mediated transcriptional activation is enhanced by the architectural factor HMGI-C.

Nucleic acids research ·Vol. 26 ·No. 6 ·1998-03-15 ·Pages 1433-9

Mantovani F, Covaceuszach S, Rustighi A, Sgarra R, Heath C, Goodwin GH, Manfioletti G

Abstract

High mobility group I proteins (HMGI, HMGY and HMGI-C) are a family of low molecular mass non-histone nuclear proteins which constitute an important component of the active chromatin structure. Two members of this family, HMGI and HMGY, have been demonstrated to contribute to the transcriptional regulation of several promoters by interacting with the DNA and with different transcription factors. On the contrary, very little is known about the third member, HMGI-C, which plays an important role during embryonic growth and in the process of cell transformation, its gene being rearranged in a large number of mesenchimal tumors. In this paper we show for the first time that HMGI-C is also able to function as architectural factor, enhancing the activity of a transcription factor, NF-kappaB, through the PRDII element of the beta-interferon enhancer. Moreover we show that this enhancement is absolutely dependent on the binding of HMGI-C to its target sequence. The demonstration that HMGI-C is able to modulate transcription is thus an important initial step in the identification of genes regulated by this factor.

MeSH Terms
3T3 Cells Animals Base Sequence Binding Sites/genetics HMGA1a Protein HMGA2 Protein High Mobility Group Proteins/genetics,metabolism Interferon-beta/genetics Mice Mutation NF-kappa B/metabolism Oligodeoxyribonucleotides/genetics Promoter Regions, Genetic Protein Binding Recombinant Proteins/genetics,metabolism Transcriptional Activation
Chemicals
HMGA2 Protein High Mobility Group Proteins NF-kappa B Oligodeoxyribonucleotides Recombinant Proteins HMGA1a Protein Interferon-beta
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mantovani F
Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, via Giorgieri, 134127 Trieste, Italy.
Covaceuszach S
Rustighi A
Sgarra R
Heath C
Goodwin G H
Manfioletti G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/26.6.1433
Published
1998-03-15
Pages
1433-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147413
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/26.6.1433 PMC Full Text View journal details
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