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PMID: 9488439 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse.

Molecular and cellular biology ·Vol. 18 ·No. 3 ·1998-03-00 ·Pages 1248-56

Korinek V, Barker N, Willert K, Molenaar M, Roose J, Wagenaar G, Markman M, Lamers W, Destree O, Clevers H

Abstract

Tcf transcription factors interact with beta-catenin and Armadillo to mediate Wnt/Wingless signaling. We now report the characterization of genes encoding two murine members of the Tcf family, mTcf-3 and mTcf-4. mTcf-3 mRNA is ubiquitously present in embryonic day 6.5 (E6.5) mouse embryos but gradually disappears over the next 3 to 4 days. mTcf-4 expression occurs first at E10.5 and is restricted to di- and mesencephalon and the intestinal epithelium during embryogenesis. The mTcf-3 and mTcf-4 proteins bind a canonical Tcf DNA motif and can complex with the transcriptional coactivator beta-catenin. Overexpression of Wnt-1 in a mammary epithelial cell line leads to the formation of a nuclear complex between beta-catenin and Tcf proteins and to Tcf reporter gene transcription. These data demonstrate a direct link between Wnt stimulation and beta-catenin/Tcf transcriptional activation and imply a role for mTcf-3 and -4 in early Wnt-driven developmental decisions in the mouse embryo.

MeSH Terms
3T3 Cells Amino Acid Sequence Animals Cell Line Cell Nucleus/metabolism Chickens Cloning, Molecular Cytoskeletal Proteins/metabolism Embryonic and Fetal Development Gene Expression Genes, Reporter HMGB Proteins Humans Luciferases/genetics Mice Molecular Sequence Data PC12 Cells Proto-Oncogene Proteins/biosynthesis,genetics,metabolism Rats Signal Transduction TCF Transcription Factors Trans-Activators Transcription Factor 7-Like 1 Protein Transcription Factor 7-Like 2 Protein Transcription Factors/biosynthesis,metabolism Transcriptional Activation Wnt Proteins Wnt1 Protein Zebrafish Proteins beta Catenin
Chemicals
CTNNB1 protein, human CTNNB1 protein, mouse Ctnnb1 protein, rat Cytoskeletal Proteins HMGB Proteins Proto-Oncogene Proteins TCF Transcription Factors TCF7L1 protein, human TCF7L2 protein, human Tcf7l1 protein, mouse Tcf7l1 protein, rat Tcf7l2 protein, mouse Tcf7l2 protein, rat Trans-Activators Transcription Factor 7-Like 1 Protein Transcription Factor 7-Like 2 Protein Transcription Factors WNT1 protein, human Wnt Proteins Wnt1 Protein Wnt1 protein, mouse Wnt1 protein, rat Zebrafish Proteins beta Catenin Luciferases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Korinek V
Department of Immunology, University Hospital, Utrecht, The Netherlands.
Barker N
Willert K
Molenaar M
Roose J
Wagenaar G
Markman M
Lamers W
Destree O
Clevers H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.3.1248
Published
1998-03-00
Pages
1248-56
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC108837
Subset
IM
Databases
GENBANK
AJ223069, AJ223070
External Links
PubMed source DOI: 10.1128/MCB.18.3.1248 PMC Full Text View journal details
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