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PMID: 947891 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Structure of Caulobacter deoxyribonucleic acid.

Journal of bacteriology ·Vol. 126 ·No. 3 ·1976-06-00 ·Pages 1305-15

Wood NB, Rake AV, Shapiro L

Abstract

The deoxyribonucleic acid of the dimorphic bacterium Caulobacter crescentus contains a component that renatures with rapid, unimolecular kinetics. This component was present in both swarmer and stalked cells and exhibited the sensitivity to endonuclease S1 expected for hairpin loops. Double-stranded side branches between 100 and 600 nucleotide pairs in length were visible in electron micrographs of rapidly reassociating deoxyribonucleic acid isolated by hydroxyapatite chromatography. No extrachromosomal elements were found in spite of systematic attempts to detect their presence. These results indicate that the rapidly reassociating fraction derives from inverted repeat sequences within the chromosome and not from cross-links or plasmids. We estimate that there are approximately 350 inverted repeat regions per Caulobacter genome. The kinetic complexity of Caulobacter deoxyribonucleic acid, however, is no greater than that of other bacteria.

MeSH Terms
Bacteria/analysis Base Sequence DNA Restriction Enzymes/metabolism DNA, Bacterial/analysis DNA, Circular/analysis Nucleic Acid Renaturation Nucleotides/analysis
Chemicals
DNA, Bacterial DNA, Circular Nucleotides DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wood N B
Rake A V
Shapiro L
References (48)
48 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.126.3.1305-1315.1976
Published
1976-06-00
Pages
1305-15
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233157
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.126.3.1305-1315.1976 PMC Full Text View journal details
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