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PMID: 946822 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Compensator transducer increases ease, accuracy, and rapidity of measuring changes in specimen birefringence with polarization microscopy.

Journal of microscopy ·Vol. 106 ·No. 1 ·1976-01-00 ·Pages 63-9

Salmon ED, Ellis GW

Abstract

An instrument has been designed to improve substantially the efficiency and convenience of measuring specimen birefringence retardation (BR) with a conventional Brace-Köhler compensator. The design is based on the precise transduction of the angular position of the compensator's Vernier dial to an easily visible red-illuminated display on a digital voltmeter. The instrument display is accurate to within 0.1 degrees over a range of +/-25 degrees in the compensator vernier dial position which is similar to the precision obtainable with an ordinary vernier and the Brace-Köhler method of measuring BR of biological specimens. For convenience in data reduction the display can also be read directly in nanometre units, with a similar precision for specimen BR in the range of 5 nm or less which is typical of birefringent fine structures in living cells.

MeSH Terms
Birefringence Microscopy, Polarization/instrumentation Transducers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Salmon E D
Ellis G W
Article Info
Journal
Journal of microscopy
Abbr.
J Microsc
ISSN
0022-2720
Published
1976-01-00
Pages
63-9
Language
English
Region
England
NLM ID
0204522
Subset
IM
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