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PMID: 9463414 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Antibody-mediated modulation of Cryptococcus neoformans infection is dependent on distinct Fc receptor functions and IgG subclasses.

The Journal of experimental medicine ·Vol. 187 ·No. 4 ·1998-02-16 ·Pages 641-8

Yuan R, Clynes R, Oh J, Ravetch JV, Scharff MD

Abstract

Coupling of an antibody response to effector cells through the Fc region of antibodies is a fundamental objective of effective vaccination. We have explored the role of the Fc receptor system in a murine model of Cryptococcus neoformans protection by infecting mice deleted for the common gamma chain of FcRs. Passive administration of an IgG1 mAb protects FcRgamma+/- mice infected with C. neoformans, but fails to protect FcRgamma-/- mice, indicating that the gamma chain acting through FcgammaRI and/or III is essential for IgG1-mediated protection. In contrast, passive administration of an IgG3 mAb with identical specificity resulted in enhanced pathogenicity in gamma chain-deficient and wild-type mice. In vitro studies with isolated macrophages demonstrate that IgG1-, IgG2a-, and IgG2b-opsonized C. neoformans are not phagocytosed or arrested in their growth in the absence of the FcRgamma chain. In contrast, opsonization of C. neoformans by IgG3 does not require the presence of the gamma chain or of FcRII, and the internalization of IgG3-treated organisms does not arrest fungal growth.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigen-Antibody Reactions Cells, Cultured Coculture Techniques Cryptococcosis/immunology Cryptococcus neoformans/growth & development Female Immunoglobulin G/classification,immunology Macrophages/immunology,microbiology Mice Mice, Inbred C57BL Phagocytosis Receptors, Fc/immunology,physiology Rosette Formation
Chemicals
Antibodies, Monoclonal Immunoglobulin G Receptors, Fc
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yuan R
Department of Cell Biology of the Albert Einstein College of Medicine, Bronx, New York, 10461, USA.
Clynes R
Oh J
Ravetch J V
Scharff M D
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.187.4.641
Published
1998-02-16
Pages
641-8
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2212141
Subset
IM
Grants
NIDDK NIH HHS · KO8 DK-02468 · United States
NIAID NIH HHS · R01 AI-35875 · United States
NCI NIH HHS · R35 CA-39838 · United States
External Links
PubMed source DOI: 10.1084/jem.187.4.641 PMC Full Text View journal details
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