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PMID: 9461468 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A contiguous 60 kb genomic stretch from barley reveals molecular evidence for gene islands in a monocot genome.

Nucleic acids research ·Vol. 26 ·No. 4 ·1998-02-15 ·Pages 1056-62

Panstruga R, Büschges R, Piffanelli P, Schulze-Lefert P

Abstract

The contiguous DNA sequence of a 60 kb genomic interval of barley chromosome 4HL has been assembled. The region harbours a single and novel gypsy -like retrotransposon, designated BAGY-1. Only three genes appear to reside in the genomic stretch. One predicts a plant homologue of ribophorin I, a subunit of the oligosaccharyltransferase-protein complex located in the rough endoplasmatic reticulum. The second is similar to the Drosophila g1 gene encoding a ring finger protein involved in developmental processes. The observed gene density is approximately 5-fold lower than in the best characterized dicot genome of Arabidopsis but 6- to 10-fold higher than expected from an equidistant gene distribution in the complex barley genome. Our data suggest that the 60 kb genomic interval represents part of a gene island, a seemingly distinctive feature of grass genomes.

MeSH Terms
Amino Acid Sequence Animals Arabidopsis/genetics Base Composition CpG Islands DNA, Plant/genetics Drosophila/genetics Gene Expression Genes, Insect Genes, Plant Genome, Plant Hordeum/genetics Humans Introns Molecular Sequence Data Retroelements Species Specificity Zea mays/genetics
Chemicals
DNA, Plant Retroelements
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Panstruga R
The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, UK.
Büschges R
Piffanelli P
Schulze-Lefert P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/26.4.1056
Published
1998-02-15
Pages
1056-62
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147355
Subset
IM
Databases
GENBANK
Y14573
External Links
PubMed source DOI: 10.1093/nar/26.4.1056 PMC Full Text View journal details
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