Home LiteratureArticle Details

PMID: 9459302 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of Myo3, a second type-II myosin heavy chain in the fission yeast Schizosaccharomyces pombe.

FEBS letters ·Vol. 420 ·No. 2-3 ·1997-12-29 ·Pages 161-6

Motegi F, Nakano K, Kitayama C, Yamamoto M, Mabuchi I

Abstract

We cloned the myo3+ gene of Schizosaccharomyces pombe which encodes a type-II myosin heavy chain. myo3 null cells showed a defect in cytokinesis under certain conditions. Overproduction of Myo3 also showed a defect in cytokinesis. Double mutant analysis indicated that Myo3 genetically interacts with Cdc8 tropomyosin and actin. Myo3 may be implicated in cytokinesis and stabilization of F-actin cables. Moreover, the function of Myo2 can be replaced by overexpressed Myo3. We observed a modest synthetic interaction between Myo2 and Myo3. Thus, Myo2 and Myo3 seem to cooperate in the formation of the F-actin ring in S. pombe.

MeSH Terms

Actins/metabolism Amino Acid Sequence Bacterial Proteins/metabolism Blotting, Southern Carrier Proteins/genetics,metabolism Cell Division/physiology Cell Nucleus Cloning, Molecular Fluorescent Dyes/metabolism Fungal Proteins/chemistry,metabolism Gene Expression Regulation, Fungal/genetics Microscopy, Fluorescence Molecular Sequence Data Myosin Heavy Chains/chemistry,metabolism Myosin Type II Myosin Type V Myosins/chemistry,metabolism Phenotype Restriction Mapping Saccharomyces cerevisiae Proteins Schizosaccharomyces/chemistry,genetics Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Tropomyosin/metabolism

Chemicals

Actins Bacterial Proteins Carrier Proteins Fluorescent Dyes Fungal Proteins MYO2 protein, S cerevisiae MYO2 protein, S pombe Myp2 protein, S pombe Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Tropomyosin Myosin Type II Myosin Type V Myosin Heavy Chains Myosins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Motegi F

Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Japan.

Nakano K

Kitayama C

Yamamoto M

Mabuchi I

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/s0014-5793(97)01510-x

Published

1997-12-29

Pages

161-6

Language

English

Region

England

NLM ID

0155157

Subset

Databases

GENBANK

AB007633

External Links

PubMed source DOI: 10.1016/s0014-5793(97)01510-x View journal details

Analysis Services

Identification of Myo3, a second type-II myosin heavy chain in the fission yeast Schizosaccharomyces pombe.

Contact

WeChat Official Account